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Human 4-1BB/TNFRSF9/CD137 Antibody

Catalog #: AF838
4 Citations 1 Review Datasheet / COA / SDS
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AF838
AF838-SP
Detection of Human 4‑1BB/TNFRSF9/ CD137 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 4-1BB/TNFRSF9/CD137 Antibody Summary

Specificity
Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137
Leu24-Gln186
Accession # Q07011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
HEK293T human embryonic kidney cell line transfected with human 4-1BB/TNFRSF9/CD137-eGFP Fusion
Simple Western
20 µg/mL
HDLM‑2 human Hodgkin's lymphoma cells
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
Agonist Activity
Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3.
The ED50 for this effect is typically ≤ 20 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells

Human 4-1BB/TNFRSF9/CD137 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human 4‑1BB/TNFRSF9/CD137 Biotinylated Antibody (Catalog # BAF838)

Standard: Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (Catalog # 838-4B)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human 4-1BB/TNFRSF9/ CD137 antibody by Western Blot. View Larger

Detection of Human 4‑1BB/TNFRSF9/ CD137 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human 4-1BB/TNFRSF9/ CD137-eGFP Fusion. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human 4-1BB/ TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody ([catalogNumber:HAF017]]). A specific band was detected for 4-1BB/ TNFRSF9/CD137-eGFP Fusion at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry 4-1BB/TNFRSF9/CD137 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

4‑1BB/TNFRSF9/CD137 in Human Tonsil. 4-1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4-1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry Detection of 4-1BB/TNFRSF9/CD137 antibody in PHA-treated Human T Cells antibody by Flow Cytometry. View Larger

Detection of 4‑1BB/TNFRSF9/CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 µg/mL PHA then stained with Goat Anti-Human 4-1BB/TNFRSF9/ CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (AB-108-C) staining.

Simple Western View Larger

Detection of Human 4‑1BB/TNFRSF9/CD137 by Simple WesternTM. Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cells, loaded at 0.2 mg/mL. A specific band was detected for 4‑1BB/TNFRSF9/CD137 at approximately 58 kDa (as indicated) using 20 µg/mL of Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the
TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells.

References
  1. Vinay, D. and B. Kwon (1998) Semin. Immunol. 10:481.
  2. Sica, G. and L. Chen (2000) Adv. Exp. Med. Biol. 465:355.
Entrez Gene IDs
3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
Alternate Names
4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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Citations for Human 4-1BB/TNFRSF9/CD137 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Anti-CD137 monoclonal antibody enhances trastuzumab-induced, natural killer cell-mediated cytotoxicity against pancreatic cancer cell lines with low human epidermal growth factor-like receptor 2 expression
    Authors: T Masu, M Atsukawa, K Nakatsuka, M Shimizu, D Miura, T Arai, H Harimoto, C Kondo, K Kaneko, S Futagami, C Kawamoto, H Takahashi, K Iwakiri
    PLoS ONE, 2018-12-31;13(12):e0200664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD.
    Authors: Hodge G, Holmes M, Jersmann H, Reynolds P, Hodge S
    BMC Pulm Med, 2014-05-15;14(0):85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. 4-1BB is superior to CD28 costimulation for generating CD8+ cytotoxic lymphocytes for adoptive immunotherapy.
    Authors: Zhang H, Snyder KM, Suhoski MM, Maus MV, Kapoor V, June CH, Mackall CL
    J. Immunol., 2007-10-01;179(7):4910-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Functional expression of 4-1BB (CD137) in the inflammatory tissue in Crohn's disease.
    Authors: Maerten P, Geboes K, De Hertogh G, Shen C, Cadot P, Bullens DM, Van Assche G, Penninckx F, Rutgeerts P, Ceuppens JL
    Clin. Immunol., 2004-09-01;112(3):239-46.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-Fr

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Human 4-1BB/TNFRSF9/CD137 Antibody
By Anonymous on 10/13/2017
Application: Flow Sample Tested: Adult kidney Species: Human