Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2295
Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry.
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Product Details
Citations (2)
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Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human 4‑1BB Ligand/TNFSF9 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) 4‑1BB Ligand, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, and rhTWEAK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 4-1BB Ligand
Arg71-Glu254
Accession # P41273
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human 4‑1BB Ligand/TNFSF9 (Catalog # 2295-4L)
Flow Cytometry
0.25 µg/106 cells
Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of 4-1BB Ligand/TNFSF9 antibody in Human Daudi cell line antibody by Flow Cytometry. View Larger

Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry. Human Daudi Burkitt lymphoma cell line was stained with Goat Anti-Human 4-1BB Ligand/TNFSF9 Biotinylated Polyclonal Antibody (Catalog # BAF2295, filled histogram) or control antibody (BAF108, open histogram), followed by PE-conjugated Streptavidin (F0040). Staining was performed usinig our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB Ligand/TNFSF9

4-1BB ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A 26 kDa soluble form of 4-1BBL is known to occur in humans. Although it is presumably generated by MMP activity, its amino acid size is currently unreported (4). The soluble form is bioactive. Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ cells, and IFN-gamma in CD8+ cells (9, 10).

References
  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
  2. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
  3. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481.
  4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  5. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  6. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  7. Michel, J. et al. (1999) Immunology 98:42.
  8. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  9. Wen, T. et al. (2002) J. Immunol. 168:4897.
  10. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
Entrez Gene IDs
8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names
41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9

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Citations for Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A human CD137�PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade
    Authors: C Geuijen, P Tacken, LC Wang, R Klooster, PF van Loo, J Zhou, A Mondal, YB Liu, A Kramer, T Condamine, A Volgina, LJA Hendriks, H van der Ma, E Rovers, S Engels, F Fransen, R den Blanke, V Zondag-van, A Basmeleh, W Bartelink, A Kulkarni, W Marissen, CY Huang, L Hall, S Harvey, S Kim, M Martinez, S O'Brien, E Moon, S Albelda, C Kanellopou, S Stewart, H Nastri, ABH Bakker, P Scherle, T Logtenberg, G Hollis, J de Kruif, R Huber, PA Mayes, M Throsby
    Nature Communications, 2021-07-21;12(1):4445.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  2. Crystal structure of the human 4-1BB/4-1BBL complex
    Authors: RN Gilbreth, VY Oganesyan, H Amdouni, S Novarra, L Grinberg, A Barnes, M Baca
    J. Biol. Chem., 2018-05-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human 4-1BB Ligand/TNFSF9 Biotinylated Antibody
By Anonymous on 03/09/2023
Application: ELISA Sample Tested: HT1080 human fibrosarcoma cell line Species: Human