Human 4-1BB Ligand/TNFSF9 Antibody

Catalog #: MAB22952 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB22952-100
MAB22952-SP

Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry.

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Human 4-1BB Ligand/TNFSF9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 2357A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 4‑1BB Ligand/TNFSF9 in Human Daudi cell line by Flow Cytometry. Human Daudi Burkitt lymphoma cell line was stained with Rabbit Anti-Human 4-1BB Ligand/TNFSF9 Mono-clonal Antibody (Catalog # MAB22952, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺ and CD8⁺ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References

Wang, C. et al. (2009) Immunol. Rev. 229:192.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
Langstein, J. et al. (1998) J. Immunol. 160:2488.
Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
Baessler, T. et al. (2010) Blood 115:3058.
Gullo, C. et al. (2010) PLoS One 5:e10845.
Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Wen, T. et al. (2002) J. Immunol. 168:4897.
Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Alternate Names

41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9