Human 11 beta-HSD2 Antibody

Catalog #: MAB8630 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8630
MAB8630-SP

Detection of Human 11 beta ‑HSD2 by Western Blot.

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All 11 beta-HSD2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human 11 beta-HSD2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human 11 beta ‑HSD2 in direct ELISA and Western Blots.

Source

Monoclonal Mouse IgG_2A Clone # 921215

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human 11 beta ‑HSD2
Met105-Arg405
Accession # P80365

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human 11 beta ‑HSD2 by Western Blot. Western blot shows lysates of human kidney tissue, human placenta tissue, and human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human 11 beta -HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 11 beta -HSD2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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11 beta ‑HSD2 in Human Kidney. 11 beta -HSD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human 11 beta -HSD2 Monoclonal Antibody (Catalog # MAB8630) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 11 beta-HSD2

The human enzyme HSD-2/11 beta-HSD2 is encoded by the HSD11B2 gene and codes for a 405 aa protein, with high expression in kidney, colon, pancreas and placenta1. HSD-2 modulates intracellular glucocorticoid levels by catalyzing the conversion of cortisol to the inactive metabolite cortisone2. HSD-2 is abundantly expressed in human placenta and controls fetal glucocorticoid levels to protect the fetus from glucocorticoid exposure. Similarly, HSD-2 expression in the distal nephron protects mineralcorticoid receptors from glucocorticoids and helps regulate blood pressure3. Exposure to major environmental pollutant like Cadmium, inhibits activity of HSD-2 which affects the synthesis of glucocorticoids in placenta and thus contribute to developing preeclamptic conditions.

Long Name

11 beta Hydroxysteroid Dehydrogenase 2

Entrez Gene IDs

3291 (Human); 15484 (Mouse); 25117 (Rat)

Alternate Names

11 betaHSD2; 11 beta-HSD2; AME; corticosteroid 11-beta-dehydrogenase isozyme 2,11-beta-HSD2; EC 1.1.1; EC 1.1.1.-; HSD11B2; HSD11KAME1; HSD2,11-DH2; hydroxysteroid (11-beta) dehydrogenase 2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; SDR9C3; short chain dehydrogenase/reductase family 9C member 3,11-beta-hydroxysteroid dehydrogenase type 2