The HT PARP In Vivo Pharmacodynamic Assay II (Catalog # 4520B-096-K) is a high-throughput, chemiluminescent ELISA designed to quantify poly (ADP-ribose) (PAR) in tissues and cultured cells. The assay employs a two-site sandwich technique in which two different anti-PAR antibodies are used to capture and detect the target analyte. This assay is useful for measuring PAR in extracts from peripheral blood mononuclear cells, cultured cells, and tissue. Additionally, this assay can be used to monitor the efficacy of Poly (ADP-ribose) Polymerase (PARP) inhibitors or anti-cancer drugs on cellular PAR formation and cancer cell cytotoxicity.
Features
- Chemiluminescent, non-radioactive format
- Amenable to high-throughput
- Broad linear dynamic range to 1000 pg/mL
- High signal-to-noise ratio
Kit Contents
- Pre-coated 96-stripwell Microplate
- PAR Standard
- PAR Monoclonal Biotinylated Detection Antibody
- Streptavidin-HRP A
- PARP PeroxyGlow™ A and B Chemiluminescent Substrates
- Cell Lysis Buffer
- DNase I
- Magnesium Cation
- WIL2-NS Cell Lysate Standards
- Antibody & Sample Diluents
- SDS
- Plate sealers
Data Example
Measurement of PAR Levels in Jurkat Cells
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The HT PARP In Vivo Pharmacodynamic Assay II (Catalog # 4520B-096-K) was used to measure poly (ADP-ribose) (PAR) levels in cellular extracts from untreated Jurkat human acute T cell leukemia cells, Jurkat cells treated with PJ34, a potent PARP inhibitor, for 1.5 hours, and in Jurkat cell extracts incubated for 30 minutes with PAR glycohydrolase (PARG), an enzyme that digests PAR. |