HT PARP in vivo Pharmacodynamic ELISA Kit II

HT PARP in vivo Pharmacodynamic ELISA Kit II Summary

Key Benefits

• Pre-coated 96 well capture antibody plates
• High signal to noise ratio
• Detection sensitivity of 10 pg/mL of PAR
• Broad linear dynamic range to 1,000 pg/mL
• Reduced inter-assay variability
• Validated assay that measures drug action on PARP in both in vivo and in vitro settings
• 96 test size

Principle of the Assay

A monoclonal antibody specific for PAR has been pre-coated onto a microplate to capture cellular PAR and PAR attached to proteins. Incubation with a PAR Monoclonal Biotinylated Detecting Antibody, followed by addition of Streptavidin-HRP and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlate with the amount of cellular PAR.

Product Specifications

• Quantitation of PAR in tissue lysates and culture cells.
• Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
• Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
• Facilitating development of PARP and PARG targeted therapeutics.

Kit Contents

Specifications

Limitations

For research use only. Not for diagnostic use.

Product Datasheets