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Goat IgG (H+L) PE-conjugated Antibody

Catalog #: F0107
19 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0107
Detection of Goat IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG (H+L) PE-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Goat IgG Primary Antibody by Flow Cytometry View Larger

Detection of Goat IgG Primary Antibody by Flow Cytometry SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) at 10 uL/106cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG (H+L) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-kappa B Signaling Pathway
    Authors: Youn SE, Jiang F, Won HY et al.
    International journal of molecular sciences
  2. ROR1 Flow Cytometry
    Authors: Vincent T Bicocca, Jeffrey W Tyner
    BIO-PROTOCOL
  3. Phosphatidylserine binding directly regulates TIM-3 function
    Authors: Courtney M. Smith, Alice Li, Nithya Krishnamurthy, Mark A. Lemmon
    Biochemical Journal
  4. Engagement of TRAIL triggers degranulation and IFN gamma production in human natural killer cells
    Authors: Johannes Höfle, Timo Trenkner, Nadja Kleist, Vera Schwane, Sarah Vollmers, Bryan Barcelona et al.
    EMBO reports
  5. Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
    Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
    bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
  6. Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation
    Authors: Defang Li, Jin Liu, Baosheng Guo, Chao Liang, Lei Dang, Cheng Lu et al.
    Nature Communications
  7. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling
    Authors: Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al.
    Journal of Cell Science
  8. Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface
    Authors: Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Erythropoietin Receptor (EPOR) Signaling in the Osteoclast Lineage Contributes to EPO-Induced Bone Loss in Mice
    Authors: Z Awida, S Hiram-Bab, A Bachar, H Saed, D Zyc, A Gorodov, N Ben-Califa, S Omari, J Omar, L Younis, JA Iden, L Graniewitz, I Gluzman, T Liron, B Raphael-Mi, A Kolomansky, M Rauner, B Wielockx, Y Gabet, D Neumann
    International Journal of Molecular Sciences, 2022-10-10;23(19):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells
    Authors: J Grau-Expós, D Perea, M Suppi, N Massana, A Vergara, MJ Soler, B Trinite, J Blanco, J García-Pér, J Alcamí, A Serrano-Mo, J Rosado, V Falcó, M Genescà, MJ Buzon
    PloS Pathogens, 2022-01-13;18(1):e1010171.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice
    Authors: Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann
    International Journal of Molecular Sciences, 2021-12-21;23(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Breast cancer exosomes contribute to pre-metastatic niche formation and promote bone metastasis of tumor cells
    Authors: X Yuan, N Qian, S Ling, Y Li, W Sun, J Li, R Du, G Zhong, C Liu, G Yu, D Cao, Z Liu, Y Wang, Z Qi, Y Yao, F Wang, J Liu, S Hao, X Jin, Y Zhao, J Xue, D Zhao, X Gao, S Liang, Y Li, J Song, S Yu, Y Li
    Theranostics, 2021-01-01;11(3):1429-1445.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Intravenous delivery of granulocyte-macrophage colony stimulating factor impairs survival in lipopolysaccharide-induced sepsis
    Authors: J Krebs, A Hillenbran, C Tsagogiorg, C Patry, B Tönshoff, B Yard, G Beck, N Rafat
    PLoS ONE, 2019-06-20;14(6):e0218602.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal
    Authors: P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon
    Cell Death Dis, 2018-09-11;9(9):927.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Deficiency of oncostatin M receptor beta (OSMRbeta) exacerbates high-fat diet-induced obesity and related metabolic disorders in mice.
    Authors: Komori T, Tanaka M, Senba E, Miyajima A, Morikawa Y
    J Biol Chem, 2014-04-02;289(20):13821-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces.
    Authors: Nam, Giri, Lee, Young-Kw, Lee, Hye Yeon, Ma, Min Jung, Araki, Masatake, Araki, Kimi, Lee, Seungbok, Lee, Im-Soon, Choi, Eun Youn
    J Immunol, 2013-10-16;191(11):5730-42.
    Applications: Secondary Antibody
  17. Lack of oncostatin M receptor beta leads to adipose tissue inflammation and insulin resistance by switching macrophage phenotype.
    Authors: Komori T, Tanaka M, Senba E, Miyajima A, Morikawa Y
    J Biol Chem, 2013-06-11;288(30):21861-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009-02-06;113(24):6120-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
    Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
    BMC Cancer, 2013-11-25;13(0):557.

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Goat IgG (H+L) PE-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody
By Yannick Alexandre on 07/06/2022
Application: Flow Sample Tested: Splenocytes Species: Human

Antibody used 1/200 in intracellular staining. Negative control with only secondary antibody is overlaid.

Goat IgG (H+L) PE-conjugated Antibody
By Anonymous on 04/29/2021
Application: Flow Sample Tested: Bone marrow cells Species: Mouse

Mouse bone marrow cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).