Feline IL-8/CXCL8 Antibody Summary
Ala23-Ala101
Accession # Q9XSX5.1
Applications
Feline IL-8/CXCL8 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Feline IL-8/CXCL8 Antibody. Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND50 is typically 0.075-0.375 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8
Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.
- Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271.
- Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508.
- Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43.
- Parhar, K. et al. (2003) Immunology 108:502.
Product Datasheets
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