Home / IgG / Goat IgG NorthernLights™ NL557-conjugated Antibody

Goat IgG NorthernLights™ NL557-conjugated Antibody

Catalog #: NL001
36 Citations 2 Reviews Datasheet / COA / SDS
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL001
Detection of Goat IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG NorthernLights™ NL557-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Goat IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL557 (Excitation= 557 nm, Emission= 574 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Goat IgG Primary Antibody by Immunocytochemistry. Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control). Detection of human EGFR by incubating cells with 5 ug/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Nonclassical monocytes potentiate anti-tumoral CD8+ T cell responses in the lungs
    Authors: Lindsey E. Padgett, Paola M. Marcovecchio, Claire E. Olingy, Daniel J. Araujo, Kathleen Steel, Huy Q. Dinh et al.
    Frontiers in Immunology
  2. Generation of a human iPSC line (CIBi014-A) from a patient with Parkinson's disease carrying a novel heterozygotic PARK8 (LRRK2) mutation
    Authors: L Li, X Si, J Yang, M Lei, H Liu, J Ruan, H Fu, W Li, H Yang, X Lei, H Sang
    Stem Cell Research, 2022-12-09;66(0):102995.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer
    Authors: J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi
    Cancer Research, 2022-11-15;0(0):OF1-OF14.
  4. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
    Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
    Stem Cell Research, 2022-10-31;65(0):102958.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Production of a human iPSC line from an early-onset Parkinson's disease patient with a novel CHCHD2 gene truncated mutation
    Authors: Z Jiang, XJ Gu, WM Su, QQ Duan, JY Lin, B Cao, HF Shang, YP Chen
    Stem Cell Research, 2022-08-04;64(0):102881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using CRISPR/Cas9 technology
    Authors: Y Sun, J Fu, J Yang, J Zhao, J Rong
    Oncogene, 2022-07-11;63(0):102859.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  7. Generation of a human iPSC line CIBi011-A from amniocytes of a healthy fetus
    Authors: Y Shi, S Wu, B Yang, B Yu, Y Yan, J Yang, M Chu, X Hu
    Stem Cell Research, 2022-05-04;62(0):102801.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Generation of a human induced pluripotent stem cell line FMUPDCi001-A from a patient with mental retardation, autosomal recessive 36 (MRT36) carrying the variants c.219dupA and c.587C�>�T in ADAT3
    Authors: X Hu, S Xiong, X Zhou, L Sun
    Stem Cell Research, 2022-04-04;61(0):102777.
    Species: Human
    Sample Types: Spheroid
    Applications: ICC
  9. CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells
    Authors: DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster
    Journal of reproductive immunology, 2022-01-14;150(0):103477.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  10. Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain
    Authors: J Zhao, C Huangfu, Z Chang, W Zhou, AT Grainger, Z Liu, W Shi
    Physiological Reports, 2021-06-01;9(11):e14829.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Generation of a human induced pluripotent stem cell line (SMUSHi001-A) from a patient with 46, XX male sex reversal syndrome carrying the SRY gene
    Authors: R Yan, S Wu, W Wang, M Chu, P Liu
    Stem Cell Research, 2021-05-24;54(0):102397.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Generation of a human iPSC line QDMHi001-A from a patient with Marfan syndrome carrying a heterozygous c.6772�T�>�C variant in FBN1
    Authors: S Wu, Z Zhang, L Wang, J Yu
    Stem Cell Research, 2021-05-16;54(0):102390.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Generation of a human iPSC line CIBi009-A from a patient with familial hypercholesterolemia carrying variants of LDLR c.T1241G and APOB c.G1618T
    Authors: W Ge, Y Song, M Chu, Y Liu, B Yang, K Wang, B Yu, C Song, Y Wang, J Yang
    Stem Cell Research, 2021-04-20;53(0):102347.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  14. FSTL1 promotes growth and metastasis in gastric cancer by activating AKT related pathway and predicts poor survival
    Authors: M Wu, Y Ding, N Wu, J Jiang, Y Huang, F Zhang, H Wang, Q Zhou, Y Yang, W Zhuo, L Teng
    American journal of cancer research, 2021-03-01;11(3):712-728.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
    Authors: M Zhou, R Wei, Y Jiang, J Fu, Y Liu, B Yang, B Yu, Y Lin, X Ran, WH Lai, M Chu, Y Hu, J Yang, HF Tse
    Stem Cell Research, 2020-12-10;50(0):102120.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Generation of a human induced pluripotent stem cell line (SBWCHi001-A) from a patient with NEDSDV carrying a pathogenic mutation in CTNNB1 gene
    Authors: R Yan, P Liu, F Li, M Chu, J Lei, F Wang, L Luo, X Xu
    Stem Cell Res, 2020-11-19;49(0):102091.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28A�>�G and IVS-II-654C�>�T in HBB
    Authors: S Wu, H Wei, M Chu, Z Weng
    Stem Cell Res, 2020-10-29;49(0):102074.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function
    Authors: MJ Munro, L Peng, SK Wickremese, ST Tan
    PLoS ONE, 2020-05-19;15(5):e0232934.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. GPR30 receptor promotes preoperative anxiety-induced postoperative hyperalgesia by up-regulating GABAA-&alpha4&beta1&delta subunits in periaqueductal gray in female rats
    Authors: M Jiang, Y Sun, Y Lei, F Hu, Z Xia, Y Liu, Z Ma, X Gu
    BMC Anesthesiol, 2020-04-22;20(1):93.
    Species: Rats
    Sample Types: Tissue
    Applications: IHC
  20. Effects of thrombospondin-4 on pro-inflammatory phenotype differentiation and apoptosis in macrophages
    Authors: Rahman MT, Muppala S, Wu J et al.
    Cell Death Dis
  21. Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
    Authors: C Lu, NE Sanjana
    Stem Cell Res, 2019-11-01;41(0):101643.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Atherogenesis in the Carotid Artery with and without Interrupted Blood Flow of Two Hyperlipidemic Mouse Strains
    Authors: Jian Zhao, Chaoji Huangfu, Zhihui Chang, Andrew T. Grainger, Zhaoyu Liu, Weibin Shi
    Journal of Vascular Research
  23. Enhanced Tropism of Species B1 Adenoviral-Based Vectors for Primary Human Airway Epithelial Cells
    Authors: Ni Li, Ashley L. Cooney, Wenli Zhang, Anja Ehrhardt, Patrick L. Sinn
    Molecular Therapy - Methods & Clinical Development
  24. Targeting Tumor Vascular CD99 Inhibits Tumor Growth
    Authors: Elisabeth J. M. Huijbers, Inge M. van der Werf, Lisette D. Faber, Lena D. Sialino, Pia van der Laan, Hanna A. Holland et al.
    Frontiers in Immunology
  25. Understanding Hematopoietic Stem Cell Development through Functional Correlation of Their Proliferative Status with the Intra-aortic Cluster Architecture
    Authors: A Batsivari, S Rybtsov, C Souilhol, A Binagui-Ca, D Hills, S Zhao, P Travers, A Medvinsky
    Stem Cell Reports, 2017-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
    Sci Rep, 2016-12-06;6(0):38278.
    Species: Human
    Sample Types: Whole Cells
    Applications: Secondary Antibody
  27. Collagen-induced arthritis and imiquimod-induced psoriasis develop independently of interleukin-33
    Authors: Sara Khaleghparast Athari
    Arthritis Res Ther, 2016-06-18;18(1):143.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Interleukin-33 from Monocytes Recruited to the Lung Contributes to House Dust Mite-Induced Airway Inflammation in a Mouse Model
    Authors: Hiroki Tashiro
    PLoS ONE, 2016-06-16;11(6):e0157571.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. A promyelocytic leukemia protein-thrombospondin 2 axis and the risk of relapse in neuroblastoma
    Authors: M Dvorkina, V Nieddu, S Chakelam, A Pezzolo, S Cantilena, AP Leite, O Chayka, T Regad, A Pistorio, AR Sementa, A Virasami, J Barton, X Montano, T Lechertier, N Brindle, D Morgenster, M LE Bras, A Burns, N Saunders, K Hodivala-D, L Bagella, H de The
    Clin Cancer Res, 2016-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.
    Authors: Avlas O, Bragg A, Fuks A, Nicholson J, Farkash A, Porat E, Aravot D, Levy-Drummer R, Cohen C, Shainberg A, Arad M, Hochhauser E
    PLoS ONE, 2015-06-01;10(6):e0120175.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Human umbilical cord and dental pulp-derived mesenchymal stem cells: Biological characteristics and potential roles in vitro and in vivo
    Authors: XIAOYAN CUI, LEI CHEN, TING XUE, JING YU, JIE LIU, YAZHONG JI et al.
    Molecular Medicine Reports
  32. Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent.
    Authors: Nischalke H, Berger C, Lutz P, Langhans B, Wolter F, Eisenhardt M, Kramer B, Kokordelis P, Glassner A, Muller T, Rosendahl J, Fischer J, Berg T, Grunhage F, Leifeld L, Soyka M, Nattermann J, Sauerbruch T, Stickel F, Spengler U
    PLoS ONE, 2013-11-18;8(11):e80848.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Functional Properties of Rare Missense Variants of Human CDH13 Found in Adult Attention Deficit/Hyperactivity Disorder (ADHD) Patients
    Authors: Thegna Mavroconstanti, Stefan Johansson, Ingeborg Winge, Per M. Knappskog, Jan Haavik
    PLoS ONE
  34. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
    Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
    J Immunol, 2013-04-15;190(10):5267-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
    Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
    Science, 2010-11-05;330(6005):827-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury.
    Authors: Abarbanell AM, Wang Y, Herrmann JL
    Am. J. Physiol. Heart Circ. Physiol., 2010-02-19;298(5):H1529-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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Reviews for Goat IgG NorthernLights™ NL557-conjugated Antibody

Average Rating: 4 (Based on 2 Reviews)

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody
By Anonymous on 02/05/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung,fixed tissue Species: Mouse

I use goat derived primary antibody to stain the fixed tissue, and this secondary antibody works great with it!

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody
By Simon Liu on 01/14/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: RT4-D6P2T rat schwannoma cell line Species: Human

RT4 cell line with Primary ecadherin-goat

Immunocytochemistry/Immunofluorescence Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody NL001