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DAPI

Catalog #: 5748
14 Citations Datasheet / COA / SDS
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5748/50
DAPI | CAS No. 28718-90-3 | Fluorescent DNA Stains
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Description: Blue-fluorescent DNA stain / dye. Used for: nuclear counterstain, fixed and live-cell staining, assessing apoptosis. Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), ISH
Alternative Names: DAPI Dye

Chemical Name: 2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride

Purity: ≥95%

Product Details
Citations (14)
Reviews
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Key information: DAPI is a widely used blue-fluorescent DNA stain / dye. Membrane impermeant to live cells. For nuclear counterstain, fixed and live-cell staining (at high concentration).

Used for: nuclear counterstain, fixed and live-cell staining (at high concentration), DNA visualization, chromosome staining, DNA staining in agarose gels, apoptosis analysis, mouse embryos or fetal organs visualization.

Application: flow cytometry, confocal microscopy, immunofluorescence (IHC, ICC), and ISH.

Properties and Photophysical Data: DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 350 nm and 470 nm, respectively.

Scientific Data

Confocal Microscopy DAPI staining View Larger

DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.

Confocal Microscopy DAPI counterstaining alongside MitoBrilliant™ Live 646 View Larger

DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.

Fluorescence Imaging RNAScope™ Assay with DAPI Counterstain View Larger

RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Optical Data for DAPI

DAPI Dye Spectra
Emission Color Blue
λabs 350 nm
λem 470 nm
Closest Laser line 405 nm
Application Flow Cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M.Wt:
350.25
Formula:
C16H15N5.2HCl
Solubility:
Soluble to 100 mM in DMSO and to 10 mM in water with gentle warming
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
28718-90-3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Imaging specific cellular glycan structures using glycosyltransferases via click chemistry.
    Wu Z, Person A, Anderson M, Burroughs B, Tatge T, Khatri K, Zou Y, Wang L, Geders T, Zaia J, Sackstein R
    Glycobiology, 2017;0(0):.
  2. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.
    Russell et al.
    Nature, 1975;253:461
  3. Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen.
    Mildner et al.
    Cancer Lett., 1978;4:89
  4. Two distinct trophectoderm lineage stem cells from human pluripotent stem cells
    A Mischler, V Karakis, J Mahinthaku, CK Carberry, A San Miguel, JE Rager, RC Fry, BM Rao
    The Journal of Biological Chemistry, 2021;0(0):100386.

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Citations for DAPI

The citations listed below are publications that use Tocris products. Selected citations for DAPI include:

14 Citations: Showing 1 - 10

  1. Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells.
    Authors: Thanaphum Et al.
    J Dent Sci  2023;18:105-111
  2. Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming.
    Authors: Yu Et al.
    Cell Mol Gastroenterol Hepatol  2023;15:1255-1275
  3. BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.
    Authors: Yelyzaveta Et al.
    Nat Commun  2023;14:1085
  4. Regulation of Cx43 Gap Junction Intercellular Communication by Bruton's Tyrosine Kinase and Interleukin-2-Inducible T-Cell Kinase.
    Authors: Paul L Et al.
    Biomolecules  2023;13
  5. Erythropoietin administration exerted neuroprotective effects against cardiac ischemia/reperfusion injury.
    Authors: Nipon Et al.
    Curr Res Pharmacol Drug Discov  2022;3:100124
  6. A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.
    Authors: Marit H Et al.
    Cell Rep  2022;39:110663
  7. Modulation of mitochondrial dynamics rescues cognitive function in rats with 'doxorubicin-induced chemobrain' via mitigation of mitochondrial dysfunction and neuroinflammation.
    Authors: Titikorn Et al.
    FEBS J  2022;289:6435-6455
  8. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance.
    Authors: Satu Et al.
    J Exp Med  2022;219
  9. HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
    Authors: Digvijay Et al.
    Science  2021;371
  10. A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.
    Authors: Elizabeth Et al.
    STAR Protoc  2021;2:100261

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