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Hoechst 33342

Catalog #: 5117
12 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5117/50
Hoechst 33342 | CAS No. 875756-97-1 | Fluorescent DNA Stains
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Description: Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy

Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride

Purity: ≥98%

Product Details
Citations (12)
Reviews (3)
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Key information: Hoechst 33342 is a blue-fluorescent dye for DNA staining. Cell permeable. Suitable for fixed and live-cell staining. Commonly used as a counterstain in fluorescence microscopy.

Used for: nuclear counterstain, DNA visualization, cell cycle studies, apoptosis analysis.

Application: flow cytometry, confocal microscopy.

Properties and Photophysical Data: Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Excitation and emission maxima (λ) are 350 nm and 461 nm, respectively.

Scientific Data

Fluorescence Imaging Fluorescent dye for staining DNA View Larger

Hoechst 33342 | CAS No. 875756-97-1 Fluorescent dye for staining DNA

Optical Data for Hoechst 33342

Hoechst 33342 Dye Spectra
Emission Color Blue
λabs 350 nm
λem 461 nm
Cell Permeable Yes
Application Flow Cytometry, Confocal Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
561.93
Formula:
C27H28N6O.3HCl
Solubility:
Soluble to 50 mM in water and to 50 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
875756-97-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Citations for Hoechst 33342

The citations listed below are publications that use Tocris products. Selected citations for Hoechst 33342 include:

12 Citations: Showing 1 - 10

  1. A Simple and Affordable Method to Create Nonsense Mutation Clones of p53 for Studying the Premature Termination Codon Readthrough Activity of PTC124.
    Authors: Ke-Fang Et al.
    Biomedicines  2023;11
  2. Methods for mitochondrial health assessment by High Content Imaging System.
    Authors: Chayanon Et al.
    MethodsX  2022;9:101685
  3. A Multiscale Study of Phosphorylcholine Driven Cellular Phenotypic Targeting.
    Authors: Diana Et al.
    ACS Cent Sci  2022;8:891-904
  4. Receptor Interactome Discovery with RDIMIS, a Membrane Protein Interaction Screen Using Recombinant Extracellular Vesicles.
    Authors: Mike Et al.
    Curr Protoc  2022;2:e483
  5. Involvement of P2Y12 receptors in a nitroglycerin-induced model of migraine in male mice.
    Authors: Mária Et al.
    Br J Pharmacol  2021;178:4626-4645
  6. Mechanical Counterbalance of Kinesin and Dynein Motors in a Microtubular Network Regulates Cell Mechanics, 3D Architecture, and Mechanosensing.
    Authors: Jian Et al.
    ACS Nano  2021;15:17528-17548
  7. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells.
    Authors: Steve R Et al.
    Cancers (Basel)  2021;13
  8. Engineering Elastic Nano- and Micro-Patterns and Textures for Directed Cell Motility.
    Authors: Paolo P Et al.
    STAR Protoc  2020;1
  9. SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance.
    Authors: Yang Et al.
    Autophagy  2019;:1
  10. GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion.
    Authors: Jie Et al.
    Autophagy  2019;15:1787-1800

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Reviews for Hoechst 33342

Average Rating: 5 (Based on 3 Reviews)

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Hoechst for nuclear staining
By Anonymous on 10/21/2021
Species: Human

Hoechst was used for nuclear staining. Add stain at 1 ug/mL and incubate cells at room temperature for 10 minutes.

Hoechst for nuclear staining 5117
staining nucleus of live cells
By Anonymous on 09/10/2020
Species: Human

I used this dye to stain the nucleus of live cells. After making the stock solution, diluted the dye 1:1000 ratio in the culture media/PBS and added to cells and incubated for 5 mins.

staining nucleus of live cells 5117
Staining nuclei
By Anonymous on 10/27/2019
Species: Human

Add Hoechst dyes at 1 ug/mL.Incubate cells at room temperature for 10 minutes.

Staining nuclei 5117
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