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Anti- Trastuzumab (Anti-Idiotype) Antibody

Catalog #: MAB95471
4 Citations Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB95471-100
MAB95471-SP
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Detection of Trastuzumab by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Anti- Trastuzumab (Anti-Idiotype) Antibody Summary

Specificity
Detects Trastuzumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2261E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Full-length Trastuzumab
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
ELISA
Detects Trastuzumab in direct ELISAs.
 
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Trastuzumab antibody by Western Blot. View Larger

Detection of Trastuzumab by Western Blot. Western blot of Centuximab (negative control), Rituximab (negative control), Trastuzumab, and Teprotumumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Trastuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB95471) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Trastuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Trastuzumab (Anti-ErbB2/Her2) on MCF-7 human cell line antibody by Flow Cytometry. View Larger

Detection of Trastuzumab (Anti-ErbB2/Her2) on MCF-7 human cell line by flow cytometry. MCF-7 Human cell line was stained with Human Trastuzumab (anti-Human ErbB2/Her2) Monoclonal Antibody (MAB9589), then Anti-Trastuzumab (Anti-Idiotype) (Catalog # MAB95471, filled histogram) or Rabbit IgG Isotype Control Antibody (MAB1050, open histogram) followed by APC-conjugated anti-rabbit IgG secondary antibody (F0111).

ELISA Specificity of Anti-Trastuzumab (Anti-Idiotype) Antibody in Direct Antibody in ELISA. View Larger

Specificity of Anti-Trastuzumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Trastuzumab (Anti-Idiotype) Antibody (Catalog # MAB95471, 333 ng/mL) detected Trastuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trastuzumab

The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Alternate Names
Trastuzumab

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Citations for Anti- Trastuzumab (Anti-Idiotype) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
    Authors: Jason T. Smith, Alena Rudkouskaya, Shan Gao, Juhi M. Gupta, Arin Ulku, Claudio Bruschini et al.
    Optica
  2. Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug–Target Engagement
    Authors: Marien Ochoa, Alena Rudkouskaya, Jason T. Smith, Xavier Intes, Margarida Barroso
    Methods in Molecular Biology

FAQs

  1. What is an anti-idiotype antibody?

    • An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody.  We have anti-idiotype antibodies to commecially available therapeutic antibodies or  biosimilars.

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