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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Catalog #: F0101B
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0101B
Detection of Mouse IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG Primary Antibody by Flow Cytometry View Larger

Detection of Mouse IgG Primary Antibody by Flow Cytometry MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calreticulin-2 Monoclonal Antibody (Catalog # MAB2927, filled histogram) or isotype control antibody (Catalog # MAB0041), open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) at 10 µL/106cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration
    Authors: Jong Min Hong, Jin-Woo Lee, Dong-Seung Seen, Jae-Yeon Jeong, Won-Ki Huh
    Cell Commun Signal
  2. Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Authors: SA Felt, GN Droby, VZ Grdzelishv
    J. Virol., 2017-07-27;0(0):.
  3. Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia
    Authors: Melody Lok-Yi Chan, Sammy Wing-Ming Shiu, Ching-Lung Cheung, Anskar Yu-Hung Leung, Kathryn Choon-Beng Tan
    Endocrine Connections
  4. Simultaneous activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell migration
    Authors: C Park, JW Lee, K Kim, DS Seen, JY Jeong, WK Huh
    Scientific Reports, 2023-02-02;13(1):1894.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Mouse
    Sample Types: Whole Cells
  6. SARS-CoV-2 accessory protein ORF8 is secreted extracellularly as a glycoprotein homodimer
    Authors: K Matsuoka, N Imahashi, M Ohno, H Ode, Y Nakata, M Kubota, A Sugimoto, M Imahashi, Y Yokomaku, Y Iwatani
    The Journal of Biological Chemistry, 2022-02-11;0(0):101724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Measles Vaccines Designed for Enhanced CD8+ T Cell Activation
    Authors: E Busch, KD Kubon, JKM Mayer, G Pidelaserr, J Albert, B Hoyler, JPW Heidbueche, KB Stephenson, BD Lichty, W Osen, SB Eichmüller, D Jäger, G Ungerechts, CE Engeland
    Viruses, 2020-02-21;12(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granuloma by inhibiting inflammation and infiltration of macrophages
    Authors: D Liu, BC Chan, L Cheng, MS Tsang, J Zhu, CW Wong, D Jiao, HY Chan, PC Leung, CW Lam, CK Wong
    Sci Rep, 2018-03-02;8(1):3903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Magnitude of viremia, antigenemia and infection of circulating monocytes in children with mild and severe dengue
    Authors: Federico Perdomo-Ce
    Acta Trop, 2016-12-13;167(0):1-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
    Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
    J. Immunol., 2011-12-16;188(3):1002-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
    Authors: Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J
    Cytotechnology, 2009-02-14;58(3):153-62.
    Species: Human
    Sample Types: Antibody
    Applications: Flow Cytometry
  12. H1/pHGFK1 nanoparticles exert anti-tumoural and radiosensitising effects by inhibition of MET in glioblastoma
    Authors: W Zhang, R Duan, J Zhang, WKC Cheung, X Gao, R Zhang, Q Zhang, M Wei, G Wang, Q Zhang, PJ Mei, HL Chen, H Kung, MC Lin, Z Shen, J Zheng, L Zhang, H Yao
    Br. J. Cancer, 2018-01-18;0(0):.

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody F0101B