CyTOF®, also known as mass cytometry, takes advantage of the low spectral overlap between heavy metal isotopes. In this technique, heavy metals are conjugated to antibodies, and their unique mass signatures are detected by time-of-flight mass spectrometry. Heavy metals take the place of fluorophores which are relatively limited by their overlap in fluorescence emission spectra. This allows the simultaneous detection and resolution of more molecular targets than are practical with the use of fluorescent flow cytometry dyes. Over 40 cellular targets can be identified on a single cell without the need for fluorescence compensation adjustments.
Benefits of R&D Systems antibodies for established conjugation methods
- Lyophilized for reconstitution at high concentration and improved conjugation efficiency
- No BSA or other carrier proteins that could interfere with conjugation
- No buffer exchange step required before conjugation
- Can also be conjugated to fluorochromes for traditional flow cytometry
R&D Systems antibodies lyophilized in a CyTOF-ready format
Search Our CyTOF-ready Antibodies
Recent Mass Cytometry/CyTOF Citations:
High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry. Nair, N. et al. Mucosal Immunol. (2016) 9: 68. Catalog #s: MAB197, MAB170
Immune profiles to predict response to desensitization therapy in highly HLA-sensitized kidney transplant candidates. Yabu, J.M. et al. PLoS ONE (2016) 11: e0153355. Catalog #s: MAB197, MAB20172
Application of mass cytometry (CyTOF) for functional and phenotypic analysis of natural killer cells. Kay, A. et al. Methods in Mol. Biol. (2016) 1441: 13. Catalog #s: MAB1844, MAB699, MAB2014, MAB2238, MAB1381
Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. Horowitz, A. et al. J Immunol (2015) 195:4524. Catalog #s: MAB138, MAB1844, MAB2014
Strain-specific human natural killer cell recognition of influenza A virus. Kronstad, L. et al. bioRxiv (2017). Catalog #s: MAB801, MAB1300, MAB1599, MAB1380, MAB1298
Single cell mass cytometry of non-small cell lung cancer cells reveals complexity of in vivo and three-dimensional models over the Petri dish. Alfoldi, R. et al. Cells (2019) 9: E1093. Catalog #s MAB2188, MAB1418
Additional CyTOF Resources
Webinar: Single-cell Profiling with Mass Cytometry: An Overview of Technology and Current Research Applications
In this webinar, you will gain insights into how you can get the most out of multiplex single-cell profiling with mass cytometry from a leading expert. While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level. These steps include experimental planning, sample and reagent preparation, validation, quality control, to data analysis.
Cell Marker Tool
An interactive tool that allows exploration of cell surface and intracellular markers.
Complete Flow Cytometry Workflow Solutions
Products to save time and achieve dependable results