"Secondary Antibody" has 96 results in Resources.
Immunohistochemistry Supplemental Reagents
Product CategoriesAntigen Retrieval Reagents To promote epitope availability and enhance immunoreactivity. Cell & Tissue Staining Kits Complete kits to support the use of primary IgG antibodies. Secondary Antibodies We offer a range of secondary antibodies for flow cytometry, immunohistochemistry, and Western blotting.
More InfoFlow Cytometry Supplemental Reagents
Product CategoriesAntibody Isotype Controls A wide selection of species-specific IgG isotype controls conjugated to a variety of labels. Secondary Antibodies We offer a range of secondary antibodies for flow cytometry, immunohistochemistry, and Western blotting. Staining Reagents for Flow Cytometry Flow Cytometry Staining Buffer (1X) (Catalog # FC001) For immunofluorescent staining of single-cell...
More InfoN-2 Plus Media Supplement
Product CategoriesN-2 Plus Media Supplement (Catalog # AR003) is a serum-free, chemically defined, concentrated media supplement formulated to provide optimal growth conditions for neural stem cell expansion. N-2 Plus is composed of Bovine Insulin, Human Transferrin, Putrescine, Selenite, and Progesterone. The supplement is supplied as a 100X concentrate in water. Components Bovine Insulin (2500...
More InfoPreventing Non-specific Staining
ProtocolsOnce tissue or cell samples have been appropriately prepared and fixed, the samples are ready to be stained. All IHC/ICC studies are dependent on specific antibody-epitope binding, which is governed by hydrophobic interactions, ionic interactions, hydrogen bonding, and other intermolecular forces. However, the same attractive forces can also result in non-specific staining, i.e. binding of the...
More InfoB Cells
Research AreasView B Cell Markers B lymphocytes (B cells) are an essential component of the humoral immune response. Produced in the bone marrow, B cells migrate to the spleen and other secondary lymphoid tissues where they mature and differentiate into immunocompetent B cells. Part of the adaptive immune system, B cells are responsible for generating antibodies to specific antigens, which they bind via B...
More InfoCell & Tissue Staining Kits
Product CategoriesGet the most out of your primary antibodies! Designed to optimize staining on cryostat, paraffin-embedded, and free-floating tissue sections, these kits provide all the reagents required to maximize your IHC experiments. Key Benefits Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted...
More InfoProtocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
ProtocolsView Graphic Protocol The following immunohistochemistry (IHC) protocol has been developed and optimized by R&D Systems’ immunohistochemistry (IHC)/immunocytochemistry (ICC) laboratory for immunofluorescence IHC experiments using frozen tissues samples This cryosection immunofluorescence protocol provides a basic guide for the fixation, cryostat sectioning, and staining of...
More InfoProtocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
ProtocolsView Graphic Protocol The following immunohistochemistry (IHC) protocol has been developed and optimized by R&D Systems IHC/ICC laboratory for fluorescent immunohistochemistry staining experiments using paraffin-embedded tissue samples. This IHC protocol provides a basic guide for the fixation, microtome sectioning, and staining of paraffin-embedded tissue samples. Each...
More InfoCell and Tissue Staining Kits
ArticleR&D Systems’ Cell and Tissue Staining Kits detect goat, mouse, and rabbit primary antibodies using HRP-DAB, HRP-AEC, or AP-BCIP/NBT enzymatic systems. The high detection sensitivity of these staining kits is achieved by using high quality secondary biotinylated antibodies and high sensitivity streptavidin (HSS) conjugated to horseradish peroxidase (HRP) or alkaline phosphatase (AP). HSS is a...
More InfoWhat is an ELISA?
Product HighlightsELISAs Enzyme Linked Immunosorbent Assays Search for ELISA & ELISA Kits Keyword: Species: ALL Human Mouse Rat Canine ELISAs are a type of immunoassay that are commonly used to quantify levels of a specific target within a sample. Samples routinely used in ELISAs include serum, plasma, cell...
More Info