"Secondary Antibody" has 96 results in Resources.

    R&D Systems offers a range of secondary antibodies for flow cytometry, immunohistochemistry, immunocytochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. On this page you can search our secondary antibodies by reactivity, IgG class, and conjugate...

With more than 6000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your total source for immunofluorescence reagents. NorthernLights Antibody/Label Catalog # Abs/Em Maxima Laser (Ex) Comparable Fluorochromes NL-493 anti-Rabbit IgG NL-493 anti-Mouse IgG NL-493 anti-Goat IgG NL-493...

NorthernLights™ fluorescent secondary antibodies and streptavidin conjugates are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy (Figure 1)....

Immunofluorescence histochemistry is a powerful tool frequently used in neuroscience for qualitative and quantitative morphological and anatomical studies. Because samples are subjected to prolonged examination under conventional fluorescence microscopes and multiple laser-scanning confocal microscopes, it is critical for fluor­escent tags to have a low susceptibility to photo­bleaching....

VisUCyte™ HRP Polymer - Cleaner, Faster IHC Results VisUCyte™ HRP Polymer is a Biotin-free detection reagent that overcomes problems associated with Avidin-Biotin detection chemistry, such as the extra quenching steps required to inhibit endogenous Biotin and Avidin staining. R&D Systems offers secondary antibodies that are conjugated to the VisUCyte™ HRP Polymer and complete staining...

Technically, IHC and ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be identified and optimized for each individual IHC/ICC study. Optimization of IHC/ICC may also require troubleshooting a variety of factors. The tables below highlight common IHC/ICC issues and provide appropriate experimental actions. For further assistance,...

For further assistance, please contact our technical service department. Observation: No Bands Observed Observation: Faint Bands (Weak Signal) Observation: Extra Bands Observation: High Background Observation: Diffuse Bands Observation: White Bands (ECL method) Observation: Patchy uneven spots all over the blot   Observation: No Bands Observed Possible...

Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against...

Technically, IHC and ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be identified and optimized for each individual IHC/ICC study. Optimization of IHC/ICC may also require troubleshooting a variety of factors. The tables below highlight common IHC/ICC issues and provide appropriate experimental actions. For further assistance,...

Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel (Catalog # SC025) The Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel is designed for the identification and characterization of human, mouse, or rat neural progenitor cells by marker expression using immunocytochemistry and flow cytometry techniques. Kit...