"HBSS" has 9 results in Resources.

Intracellular Flow Cytometry Overview Flow cytometry can be used to stain a wide range of intracellular molecules. Cells will first need to be fixed in suspension and then permeabilized before the detection antibody is added to successful analyze intracellular targets by flow cytometry. The process of fixation and permeabilization allows the antibody to pass through the plasma membrane and...

Intracellular Flow Cytometry Overview Flow cytometry can be used to analyze various intracellular molecules including phosphorylated signaling proteins and cytokines. To stain intracellular molecules, the cells need to be fixed in suspension and then permeabilized before the detection antibody is added. This fixation/permeabilization treatment allows the antibody to pass through the plasma...

This protocol must be read in its entirety before using Catalog # NSC002 and AR003. Caution: The mouse cortical stem cells used in this protocol contain trace amounts of human transferrin and DMSO, and the media used in this protocol contains trace amounts of transferrin. The transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti HIV-1/2 and...

Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. Ready-to-use primary cortical stem cells, isolated from E14.5 Sprague-Dawley rats (Catalog # NSC001), can be grown in monolayer, as described here, or as neurospheres. These cells retain capacity for multi-lineage differentiation into astrocytes, neurons, and...

This protocol must be read in its entirety before using Catalog # NSC001. Caution: The rat cortical stem cells used in this protocol contain trace amounts of human transferrin and DMSO, and the media used in this protocol contains trace amounts of transferrin. The transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for...

Flow Cytometry Cell Viability Overview Flow cytometry provides a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating the physiological state of cells, such as in response to cytotoxic drugs and environmental factors, or during the progression of cancer and other disease states. In addition, it is often necessary...

The colony forming cell (CFC) assay, also referred to as the methylcellulose assay, is an in vitro assay used in the study of hematopoietic stem cells. The assay is based on the ability of hematopoietic progenitors to proliferate and differentiate into colonies in a semi-solid media in response to cytokine stimulation. The colonies formed can be enumerated and characterized according to their...

  Flow Cytometry Cell Viability Overview Flow cytometry is a quick and reliable method to quantify viable cells. Determining cell viability is an important step when evaluating a cells response to drug treatments or other environmental factors. It is also often necessary to distinguish dead cells in a cell suspension in order to exclude them from analysis. Dead cells can...

  Microglia are the resident immune cells of the central nervous system. They are important for maintaining homeostasis, monitoring tissue debris and pathogens, and eliminating or remodeling synapses. These cells are activated by neural injury and disease states, causing them to undergo morphological and proliferative changes, increase their phagocytic activity, and secrete...