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A reaction mixture containing PAPS, an acceptor substrate, and IMPAD1 is added to each well of a 96-well microplate. |
The reaction is initiated by adding the sulfotransferase to each well of the microplate. |
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The Malachite Green Phosphate Detection Reagents are added to the wells. The reactions are terminated and a green color develops in proportion to the amount of inorganic phosphate released by the coupling phosphatase. The absorbance of the color at 620 nm is measured. |
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