This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.
- 00:10 CFC Assay Overview
- 00:54 Aliquoting the Media
- 02:06 Preparing the Cells
- 04:05 Plating the Cells
- 05:14 Counting the Cells
- 05:53 Tips for Colony Identification
Full Written Protocols for Our CFC Assays
The Human Colony Forming Cell (CFC) Assay
The Mouse/Rat Colony Forming Cell (CFC) Assay
The Mouse Pre-B Colony Forming Cell (CFC) Assay
Guides to Choosing Methylcellulose Media
For the Human CFC Assay
For the Mouse CFC Assay
For the Rat CFC Assay