The fluorogenic peptide substrate Mca-Y-V-A-D-A-P-K(Dnp)-OH (R&D Systems, Catalog # ES007) can be used to measure the activity of certain endopeptidases and carboxypeptidases including several R&D Systems enzymes (Table 1). When an active protease cleaves the substrate at a peptide bond between the Mca (fluorophore) and Dnp (quencher) the fluorescence will increase. The increase in fluorescence can be quantified on a fluorescence plate reader and correlates with the amount of protease activity.
Table 1. R&D Systems Enzymes |
|
Recombinant Enzyme | Catalog # |
Human ACE-2 | 933-ZN |
Human BMP-1/PCP | 1927-ZN |
Human Meprin alpha Subunit/MEP1A | 3220-ZN |
Mouse ACE-2 | 3437-ZN |
Note: The materials vary depending on the enzyme being assayed.
Recombinant Enzyme | Alternate Name | Catalog # |
Human ACE-2 | ACEH | 933-ZN |
Human BMP-1/PCP | Procallagen C-proteinase (PCP) | 1927-ZN |
Human Meprin alpha Subunit/MEP1A | 3220-ZN | |
Mouse ACE-2 | ACEH | 3437-ZN |
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