The following is a suggested protocol that can be used to adapt our DuoSet ELISA Kits from their traditional 96-well plate format to a 384-well plate format. This transition increases the capacity for sample analysis, allowing more than 160 samples to be analyzed in duplicate per plate, compared to 40 samples analyzed in duplicate per plate with the traditional 96-well assay.
The ELISA sample collection and storage conditions listed are intended as general guidelines. Specific protocols may vary by cell line or tissue type. If storing sample for extended length of time, testing sample stability is recommended. Avoid repeated freeze-thaw cycles for all sample types.
Accomplish more in your day without compromising quality. The Quantikine QuicKit ELISAs provide quick, accurate quantitation of proteins in serum, plasma, and cell culture supernates. Unlike traditional ELISA kits, QuicKit ELISAs have a fast, simplified protocol that only takes 90 minutes to results with just one wash step. This video provides a general step-by-step protocol for running R&D Systems Quantikine™ QuicKit™ ELISA. Please refer to your kit booklet for specific instructions for your assay.
This video is a general step-by-step guide to running an R&D Systems DuoSet™ ELISA. Please refer to your assay insert and Certificate of Analysis for specific instructions for your assay. If you have any questions or concerns about running your DuoSet ELISA please contact R&D Systems Technical Service at techsupport@bio-techne.com.
R&D Systems Quantikine™ ELISA Kits are complete, fully validated, ready-to-run ELISAs that are designed to measure proteins in complex sample types. This video is a general step-by-step guide to running a Quantikine ELISA.
If you have any questions or concerns about running your Quantikine ELISA please contact R&D Systems Technical Service at techsupport@bio-techne.com. Please refer to your kit booklet and Certificate of Analysis for specific instructions for your assay.
Researchers can encounter a variety of problems when running an ELISA. Explore this page to understand the potential source of your problem and the steps we recommend for correcting the issue to help set you on a path for running a successful ELISA. For further assistance, please contact our technical service department.