Y-27632 in solution New

Biological Activity

Y-27632 in solution is a convenient and ready-to-use 10 mM sterile-filtered solution of Y-27632 (Cat. No. 1254), pre-dissolved in water. Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as a component of growth media for urothelial organoids and in a protocol to generate brain organoids from human iPSCs.

Supplied in vials containing a minimum of 1 mL of Y-27632 solution.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition.
   Wilson et al.
   Tissue.Eng.Part C.Methods, 2016;22:1085
2. Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling.
   Ichikawa et al.
   Cryo Letters., 2011;32:516

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