TPEN
Chemical Name: N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine
Purity: ≥97%
Biological Activity
TPEN is a heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK1 cells; acts as an intracellular chelator of proteomic Zn2+. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Also inhibits RNA binding protein Lin28 (IC50 = 2.5 μM). Cell permeable.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors.
Chimienti et al.
Biochem.Pharmacol., 2001;62:51 -
Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1.
Rana et al.
J.Inorg.Biochem., 2008;102:489 -
Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2.
Matias et al.
J.Fluoresc., 2010;20:377 -
Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells.
Hyun et al.
Invest.Opthalmol.Vis.Sci., 2001;42:460 -
Small-molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28.
Wang et al.
Cell Rep., 2018;23:3091
Product Datasheets
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Citations for TPEN
The citations listed below are publications that use Tocris products. Selected citations for TPEN include:
3 Citations: Showing 1 - 3
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Curcumin attenuates hydroxychloroquine-mediated apoptosis and oxidative stress via the inhibition of TRPM2 channel signalling pathways in a retinal pigment epithelium cell line.
Authors: Mustafa Et al.
Graefes Arch Clin Exp Ophthalmol 2023;
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The Role of Transient Receptor Potential A1 and G Protein-Coupled Receptor 39 in Zinc-Mediated Acute and Chronic Itch in Mice.
Authors: Tong Et al.
Front Mol Neurosci 2022;14:768731
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Zinc ion flux during mammalian sperm capacitation.
Authors: Kerns
Nat Commun 2018;9(1):2061
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