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TPEN

Catalog #: 4309
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4309/100
TPEN | CAS No. 16858-02-9 | Buffers, Chelators and Reagents
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Description: Cell-permeable Zn2+ chelator; also inhibits RNA binding protein Lin28

Chemical Name: N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine

Purity: ≥97%

Product Details
Citations (3)
Reviews
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

TPEN is a heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK1 cells; acts as an intracellular chelator of proteomic Zn2+. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Also inhibits RNA binding protein Lin28 (IC50 = 2.5 μM). Cell permeable.

Technical Data

M.Wt:
424.54
Formula:
C26H28N6
Solubility:
Soluble to 25 mM in DMSO and to 100 mM in ethanol
Purity:
≥97%
Storage:
Store at +4°C
CAS No:
16858-02-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors.
    Chimienti et al.
    Biochem.Pharmacol., 2001;62:51
  2. Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1.
    Rana et al.
    J.Inorg.Biochem., 2008;102:489
  3. Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2.
    Matias et al.
    J.Fluoresc., 2010;20:377
  4. Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells.
    Hyun et al.
    Invest.Opthalmol.Vis.Sci., 2001;42:460
  5. Small-molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28.
    Wang et al.
    Cell Rep., 2018;23:3091

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Citations for TPEN

The citations listed below are publications that use Tocris products. Selected citations for TPEN include:

3 Citations: Showing 1 - 3

  1. Curcumin attenuates hydroxychloroquine-mediated apoptosis and oxidative stress via the inhibition of TRPM2 channel signalling pathways in a retinal pigment epithelium cell line.
    Authors: Mustafa Et al.
    Graefes Arch Clin Exp Ophthalmol  2023;
  2. The Role of Transient Receptor Potential A1 and G Protein-Coupled Receptor 39 in Zinc-Mediated Acute and Chronic Itch in Mice.
    Authors: Tong Et al.
    Front Mol Neurosci  2022;14:768731
  3. Zinc ion flux during mammalian sperm capacitation.
    Authors: Kerns
    Nat Commun  2018;9(1):2061

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