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Thromboxane B2 Parameter Assay Kit

Catalog #: KGE011
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
KGE011
Control Products Available
QC179: Parameter Immunoassay Control Set 931 for Thromboxane B2
Multi-species Thromboxane B2 ELISA Standard Curve
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Product Details
Procedure
Citations (13)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Thromboxane B2 Parameter Assay Kit Summary

Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
0.31 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Plasma, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Measures TXB2 levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Parameter TXB2 Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure TXB2 in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 3.87 6.95 13.2 3.86 7.05 14.4
Standard Deviation 0.23 0.38 0.51 0.34 0.45 0.74
CV% 5.9 5.5 3.9 8.9 6.4 5.1

Recovery

The recovery of TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 88-110
EDTA Plasma (n=4) 94 86-105
Heparin Plasma (n=4) 97 87-107
Serum (n=4) 98 86-111
Urine (n=4) 98 84-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TXB2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Multi-species Thromboxane B2 ELISA Linearity

Scientific Data

N/A Multi-species Thromboxane B2 ELISA Standard Curve View Larger

Multi-species Thromboxane B2 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thromboxane B2

Thromboxane A2 (TXA2), is an eicosanoid involved in platelet aggregation, vasoconstriction, and reproductive functions. However, as TXA2 is rapidly degraded in vivo, TXA2 production is typically examined by assaying the more stable Thromboxane B2 (TXB2) or 11-dehydro-TXB2 (11-d-TXB2) derivatives. Levels of these metabolites are helpful markers in the study of diseases including liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and others.

Long Name:
Thromboxane B2
Alternate Names:
Thromboxane B2; TXB2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. Calibrator Diluent
  3.   Add 150 µL of Calibrator Diluent to the non-specific binding (NSB) wells.
  4.   Add 100 µL of Calibrator Diluent to the zero standard (B0) wells.
    5. 100 µL Standard, Control, or Sample
  5.   Add 100 µL of Standard, control, or sample to the remaining wells.
    6. 50 µL Primary Antibody Solution
  6.   Add 50 µL of Primary Antibody Solution to each well (except the NSB wells).
  7.   Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker. Do not wash the plate.
    8. 50 µL Conjugate
  8.   Add 50 µL of Conjugate to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on the shaker.
  9.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    11. 100 µL Stop Solution
  11. Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Thromboxane B2 Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Anti-C5a antibody vilobelimab treatment and the effect on biomarkers of inflammation and coagulation in patients with severe COVID-19: a substudy of the �phase 2�PANAMO trial
    Authors: EHT Lim, APJ Vlaar, LDJ Bos, LA van Vught, AMT Boer, RWG Dujardin, M Habel, Z Xu, MC Brouwer, D van de Bee, S de Bruin, Amsterdam
    Respiratory Research, 2022-12-24;23(1):375.
    Species: Human
    Sample Types: Plasma
  2. High-Intensity Exercise Training Improves Basal Platelet Prostacyclin Sensitivity and Potentiates the Response to Dual Anti-Platelet Therapy in Postmenopausal Women
    Authors: KA Wickham, LB Nørregaard, MH Lundberg S, SS Cheung, Y Hellsten
    Biomolecules, 2022-10-17;12(10):.
    Species: Human
    Sample Types: EDTA Plasma
  3. Serum thromboxane B2 but not soluble P-selectin levels identify ischemic stroke patients with persistent platelet reactivity while on aspirin therapy
    Authors: P Sundaravad, R Christophe, N Ramanujam, SR Chandra
    Thrombosis Research, 2021-10-28;208(0):92-98.
    Species: Human
    Sample Types: Serum
  4. Danggui-Shaoyao-San improves cognitive impairment through inhibiting O-GlcNAc-modification of estrogen alpha receptor in female db/db mice
    Authors: JJ Shi, HF Liu, T Hu, X Gao, YB Zhang, WR Li, Q Wang, SJ Zhang, D Tang, YB Chen
    Journal of ethnopharmacology, 2021-08-23;281(0):114562.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Treatment Effects of Jinlingzi Powder and Its Extractive Components on Gastric Ulcer Induced by Acetic Acid in Rats
    Authors: X Zhao, J Li, Y Meng, M Cao, J Wang
    Evid Based Complement Alternat Med, 2019-01-03;2019(0):7365841.
    Species: Rat
    Sample Types: Plasma
  6. Diverse associations between oxidative stress and thromboxane A2 in hypertensive glomerular injury
    Authors: Y Nakano, Y Nakatani, M Takami, Y Taniyama, S Arima
    Hypertens. Res., 2018-12-13;0(0):.
    Species: Rat
    Sample Types: Urine
  7. Different aspects of platelet evaluation in dengue: measurement of circulating mediators, ability to interact with the virus, the degree of activation and quantification of intraplatelet protein content
    Authors: TA da Costa B, D de Oliveir, AT de Carvalh, NR da Costa F, DF Barreto-Vi, F Cunha Jáco, OM Barth, RMR Nogueira, PC da Costa N, DC de Souza M, EL de Azeredo, PV Damasco, RV da Cunha, LM de-Oliveir
    Virus Res., 2018-09-30;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Influence of citrate concentration on the activation of blood cells in an in vitro dialysis setup
    Authors: J Gubensek, K Strobl, S Harm, R Weiss, T Eichhorn, J Buturovic-, V Weber, J Hartmann
    PLoS ONE, 2018-06-13;13(6):e0199204.
    Species: Human
    Sample Types: Plasma
  9. Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica
    Authors: D Gogoi, N Arora, B Kalita, R Sarma, T Islam, SS Ghosh, R Devi, AK Mukherjee
    Sci Rep, 2018-04-18;8(1):6210.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Acetylsalicylic acid differentially limits the activation and expression of cell death markers in human platelets exposed to Staphylococcus aureus strains
    Authors: A Chabert, P Damien, PO Verhoeven, F Grattard, P Berthelot, F Zeni, L Panicot-Du, S Robert, F Dignat-Geo, MA Eyraud, B Pozzetto, B Payrastre, F Cognasse, O Garraud, H Hamzeh-Cog
    Sci Rep, 2017-07-17;7(1):5610.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Directed transport of neutrophil-derived extracellular vesicles enables platelet-mediated innate immune response
    Nat Commun, 2016-11-15;7(0):13464.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Anti-tumor and Anti-angiogenic Effects of Aspirin-PC in Ovarian Cancer
    Mol Cancer Ther, 2016-09-16;0(0):.
    Species: Mouse
    Sample Types: Serum
  13. In vivo modulation of the inflammatory response by nonsteroidal antiinflammatory drug-related compounds that trigger L-selectin shedding.
    Authors: Herrera-Garcia A, Dominguez-Luis M, Arce-Franco M, Lopez-Fernandez J, Feria M, Barreiro O, Sanchez-Madrid F, Diaz-Gonzalez F
    Eur J Immunol, 2012-10-26;43(1):55-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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