Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge

Catalog #: SPCKB-RP-000548 Datasheet / COA / SDS

Simple Plex Rat TIM-1/KIM-1/HAVCR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-RP-000548
SPCKA-RP-000548
SPCKC-RP-000548

Control Products Available

898395: Simple Plex Control for Rat TIM-1/KIM-1/HAVCR

Simple Plex Cartridge Kit for 72 samples, 1 analyte for use with Rat Plasma / Serum.

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Rat TIM-1/KIM-1/HAVCR Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Rat Serum (10 uL), Rat EDTA Plasma (10 uL), Rat Urine (10 uL)

Sensitivity

2.89 pg/mL

Assay Range

7.1 - 10,770 pg/mL (Cell Culture Supernates, Rat Serum, Rat EDTA Plasma, Rat Urine)

Specificity

Natural and recombinant rat TIM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of rat T Cell Immunoglobulin Mucin (TIM-1) in serum, EDTA plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Rat Serum, Rat EDTA Plasma, Rat Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	21	19
Mean (pg/mL)	53.5	2476	50.9	2593
Standard Deviation	2.8	176	4.57	214
CV%	5.2	7.1	9	8.3

Recovery

Sample Type	Average % Recovery	Range %
Rat EDTA Plasma (n=4)	102	94-113
Rat Serum (n=4)	103	94-111
Rat Urine (n=4)	110	103-115

Linearity

Samples containing and/or spiked with high concentrations of rat TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Rat TIM-1/KIM-1/HAVCR Simple Plex Assay Standard Curve

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Rat TIM-1/KIM-1/HAVCR Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Long Name:

T Cell Immunoglobulin Mucin-1

Entrez Gene IDs:

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Alternate Names:

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1