Simple Plex Mouse Erythropoietin/EPO Cartridge
Simple Plex Mouse Erythropoietin/EPO Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 75 | 75 |
| Mean (pg/mL) | 77 | 3917 | 91.6 | 4348 |
| Standard Deviation | 2.13 | 96.2 | 10.8 | 363 |
| CV% | 2.8 | 2.5 | 11.8 | 8.3 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 103-103 |
| EDTA Plasma (n=4) | 94 | 89-100 |
| Heparin Plasma (n=4) | 83 | 77-90 |
| Serum (n=4) | 100 | 92-106 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Erythropoietin/EPO
Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.
The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
FAQs
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