Simple Plex Mouse Angiopoietin-like 3 Cartridge

Catalog #: SPCKB-MP-007716 Datasheet / COA / SDS

Simple Plex Mouse Angiopoietin-like 3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-007716
SPCKA-MP-007716
SPCKC-MP-007716

Control Products Available

897058: Simple Plex Control for Mouse ANGPTL3

Mouse Angiopoietin-like 3 Simple Plex Assay

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All Angiopoietin-like Protein 3/ANGPTL3 Simple Plex Assays

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Summary Precision Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse Angiopoietin-like 3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (5 uL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)

Sensitivity

5.66 pg/mL

Assay Range

15.7 - 240,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse Angiopoietin-like 3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse ANGPTL3 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	60	60
Mean (pg/mL)	146	7688	136	7884
Standard Deviation	6.51	505	16	932
CV%	4.5	6.6	11.7	11.8

Linearity

Samples containing and/or spiked with high concentrations of mouse ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Mouse Angiopoietin-like 3 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Alternate Names:

AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2