Simple Plex Human KGF/FGF-7 Cartridge

Catalog #: SPCKB-PS-001015 Datasheet / COA / SDS

Simple Plex Human KGF/FGF-7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001015
SPCKA-PS-001015
SPCKC-PS-001015

Control Products Available

898734: Simple Plex Control for Human KGF/FGF-7

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human KGF/FGF-7 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (13 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)

Sensitivity

0.18 pg/mL

Assay Range

1.8 - 6,850 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human KGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human KGF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	17
Mean (pg/mL)	41.9	2042	40.8	2122
Standard Deviation	1.79	144	3.1	125
CV%	4.3	7.1	7.6	5.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	106	97-113
EDTA Plasma (n=4)	99	92-104
Heparin Plasma (n=4)	96	92-99
Serum (n=4)	107	100-110

Linearity

Samples containing and/or spiked with high concentrations of human KGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human KGF Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.

Long Name:

Keratinocyte Growth Factor

Entrez Gene IDs:

2252 (Human); 403915 (Canine)

Alternate Names:

FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)