Simple Plex Human IL-6 R alpha Cartridge

Catalog #: SPCKB-PS-000505 Datasheet / COA / SDS
Simple Plex Human IL-6R alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000505
SPCKA-PS-000505
SPCKC-PS-000505
Control Products Available
Human IL-6 R alpha  Ella Assay
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Simple Plex Human IL-6 R alpha Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
0.214 pg/mL
Assay Range
0.8 - 2,976 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-6 R alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Interleukin 6 Receptor alpha (IL-6 R alpha ) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin), and urine.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 10 10
Mean (pg/mL) 46.5 2131 44.6 2219
Standard Deviation 1.63 173 1.97 85
CV% 3.5 8.1 4.4 3.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=3) 97 94-101

Linearity

Samples containing and/or spiked with high concentrations of human IL-6 R alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human IL-6 R alpha  Ella Assay Linearity

Scientific Data

Human IL-6 R alpha Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6R alpha

IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.

Long Name:
Interleukin 6 Receptor alpha
Entrez Gene IDs:
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names:
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

FAQs

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