Simple Plex Human CCL7/MCP-3 Cartridge

Catalog #: SPCKB-PS-000477 Datasheet / COA / SDS

Simple Plex Human CCL7/MCP-3/MARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000477
SPCKA-PS-000477
SPCKC-PS-000477

Control Products Available

898536: Simple Plex Control for Human CCL7/MCP-3/MARC

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CCL7/MCP-3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.41 pg/mL

Assay Range

0.4 - 1,550 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MCP-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Monocyte Chemotactic Protein 3 (MCP-3) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	13	13
Mean (pg/mL)	9.31	492	9.18	47.4
Standard Deviation	0.482	30.6	0.625	43.4
CV%	5.2	6.2	6.8	9.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	116	111-121
EDTA Plasma (n=4)	99	93-105
Heparin Plasma (n=4)	90	85-96
Serum (n=4)	92	81-105

Linearity

Samples containing and/or spiked with high concentrations of human MCP-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human CCL7/MCP-3 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL7/MCP-3/MARC

Human MCP-3 and mouse MARC are members of the CC subfamily of chemokines and are monocyte chemoattractants. Sequence comparisons suggest that MARC may be the mouse homolog of the human MCP-3 gene. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene.

Entrez Gene IDs:

6354 (Human); 20306 (Mouse)

Alternate Names:

C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)