Simple Plex HIV-1 p24 2nd Gen Cartridge

Catalog #: SPCKB-CS-009216 Datasheet / COA / SDS

Simple Plex HIV-1 p24 2nd Gen assay kit for use on Ella instrument. Contains cartridge, sample diluent SD30, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKA-CS-009216
SPCKC-CS-009216
SPCKB-CS-009216
ST01B-CS-009216
ST01C-CS-009216

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897653: Simple Plex Control for Human HIV-1 p24 2nd Gen

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex HIV-1 p24 2nd Gen Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL)

Sensitivity

5.1 pg/mL

Assay Range

11.7 - 112,000 pg/mL (Cell Culture Supernates)

Specificity

This assay recognizes HIV-1 p24.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of HIV-1 p24 in cell culture supernatant (CCS).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	29
Mean (µL)	782	36099	856	37492
Standard Deviation	38	2120	93.1	2511
CV%	4.9	5.9	10.9	6.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media Pretreated (n=7)	101	91-111
Cell Culture Media Untreated (n=7)	104	90-115

Linearity

Samples containing and/or spiked with high concentrations of HIV-1 p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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HIV-1 p24 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name:

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Entrez Gene IDs:

155030 (Viral)

Alternate Names:

CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)