Simple Plex Cell Activation Panel 2

Catalog #: ST01C-CS-007366 Datasheet / COA / SDS
Simple Plex 32x4 panel containing Granzyme B, IFN-gamma 3rd Gen, Perforin, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture)
Catalog # Availability Size / Price Qty
ST01C-CS-007366
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Simple Plex Cell Activation Panel 2 Summary

The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN-gamma, Perforin, and TNF-alpha in Cell Culture Supernates.

 
Sample Type & Volume Required Per Well Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity GRANB 1.31 pg/mL; IFN-gamma 0.17 pg/mL; Perforin 3.14 pg/mL; TNF-alpha 0.3 pg/mL
Assay Range GRANB 1.31 - 5,000 pg/mL; IFN-gamma 0.17 - 4,000 pg/mL; Perforin 3.14 - 12,000 pg/mL; TNF-alpha 0.3-1,160 pg/mL

Product Specifications

Reactivity Human
Specificity Natural and recombinant human GRANB, IFN-gamma, Perforin, and TNF-alpha.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
GRANB n 16 16 16 16
Mean (pg/mL) 28 1357 28.7 1377
Standard Deviation 1.26 47.1 2.87 143
CV (%) 4.5 3.5 10 10.4
IFN-gamma 3rd Gen n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV (%) 7 2.8 10.8 8.3
Perforin n 16 16 16 16
Mean (pg/mL) 92.9 3901 90.7 4145
Standard Deviation 2.07 74.9 7.41 371
CV (%) 2.2 1.9 8.2 8.9
TNF alpha 2nd Gen n 16 16 16 16
Mean (pg/mL) 6.99 383 0.662 36.9
Standard Deviation 0.345 20.6 7.81 408
CV (%) 4.9 5.4 8.5 9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
GRANB EDTA Plasma (n=4) 104 91-117
Heparin Plasma (n=4) 95 86-107
Serum (n=4) 105 91-119
IFN-gamma Cerebrospinal Fluid (n=6) 94 91-98
EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 95 83-102

Specifications

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
Simple Plex Automated Immunoassay

Product Datasheets

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Assay Procedure

GRANB, IFN-gamma, Perforin, and TNF-alpha

FAQs

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