Simple Plex Cell Activation Panel 2

Catalog #: ST01C-CS-007366 Datasheet / COA / SDS

Simple Plex 32x4 panel containing Granzyme B, IFN-gamma 3rd Gen, Perforin, and TNF-alpha 2nd Gen with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with CS (cell culture)

Catalog #	Availability	Size / Price	Qty
ST01C-CS-007366

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Summary Product Datasheets

Simple Plex Cell Activation Panel 2 Summary

The Simple Plex Human Multianalyte Assay is for the detection of human GRANB, IFN-gamma, Perforin, and TNF-alpha in Cell Culture Supernates.

Sample Type & Volume Required Per Well	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity	GRANB 1.31 pg/mL; IFN-gamma 0.17 pg/mL; Perforin 3.14 pg/mL; TNF-alpha 0.3 pg/mL
Assay Range	GRANB 1.31 - 5,000 pg/mL; IFN-gamma 0.17 - 4,000 pg/mL; Perforin 3.14 - 12,000 pg/mL; TNF-alpha 0.3-1,160 pg/mL

Product Specifications

Reactivity	Human
Specificity	Natural and recombinant human GRANB, IFN-gamma, Perforin, and TNF-alpha.
Cross-reactivity	<0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

		Intra-Assay Precision		Inter-Assay Precision
	Sample	1	2	1	2
GRANB	n	16	16	16	16
	Mean (pg/mL)	28	1357	28.7	1377
	Standard Deviation	1.26	47.1	2.87	143
	CV (%)	4.5	3.5	10	10.4
IFN-gamma 3rd Gen	n	16	16	16	16
	Mean (pg/mL)	21.1	1175	23.4	1192
	Standard Deviation	1.48	33.4	2.52	99.4
	CV (%)	7	2.8	10.8	8.3
Perforin	n	16	16	16	16
	Mean (pg/mL)	92.9	3901	90.7	4145
	Standard Deviation	2.07	74.9	7.41	371
	CV (%)	2.2	1.9	8.2	8.9
TNF alpha 2nd Gen	n	16	16	16	16
	Mean (pg/mL)	6.99	383	0.662	36.9
	Standard Deviation	0.345	20.6	7.81	408
	CV (%)	4.9	5.4	8.5	9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

	Sample Type	Average % Recovery	Range %
GRANB	EDTA Plasma (n=4)	104	91-117
	Heparin Plasma (n=4)	95	86-107
	Serum (n=4)	105	91-119
IFN-gamma	Cerebrospinal Fluid (n=6)	94	91-98
	EDTA Plasma (n=4)	89	88-90
	Heparin Plasma (n=4)	90	83-99
Serum (n=4)	95	83-102