SARS-CoV-2 ORF3a Antibody

Detection of SARS-CoV-2 ORF3a by Western Blot. Western blot shows recombinant SARS-CoV-2 ORF3a peptide. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ORF3a at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

ORF3a in HEK293 Human Cell line Transfected with SARS-CoV-2 ORF3a. ORF3a was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Mouse Anti-SAR-CoV-2 ORF3a Monoclonal Antibody (Catalog # MAB10706) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of SARS-CoV-2 ORF3a by Simple Western^TM. Simple Western lane view shows recombinant SARS-CoV-2 ORF3a, loaded at 0.2 mg/mL. A specific band was detected for ORF3a at approximately 34 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.