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Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF

Catalog #: 1088-B2
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1088-B2-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with biotinylated rmEphrin-B2/Fc Chimera (R&D Systems, Catalog # BT496) for binding with immobilized rmEph-B2/Fc Chimera in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived zebrafish Ephrin-B2 protein
Zebrafish Ephrin-B2
(Leu25 - Ala 222)
Accession # O73874		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
O73874
N-terminal Sequence
Analysis
Leu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48.5 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

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1088-B2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1088-B2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1, is a 40 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B2 preferentially interacts with receptors in the EphB family (1, 2). Mature zebrafish Ephrin-B2 consists of a 201 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 87 aa cytoplasmic domain (3). Within the ECD, zebrafish Ephrin-B2 shares 56% aa sequence identity with human, mouse, and rat Ephrin-B2. Ephrin-B2 is expressed presynaptically on neurons (4, 5). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (4 - 6). Ephrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (7, 8). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (7 - 9). Ephrin-B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (10, 11). It is upregulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (12 - 14). It functions as a cellular entry receptor for Hendra and Nipah viruses (15). Ephrin-B2 is also important for the separation of the urinary and intestinal tracts during development (16).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Durbin, L. et al. (1998) Genes Dev. 12:3096.
  4. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  5. Zhao, J. et al. (2010) Mol. Pain 6:77.
  6. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  7. Foo, S.S. et al. (2006) Cell 124:161.
  8. Wang, Y. et al. (2010) Nature 465:483.
  9. Sawamiphak, S. et al. (2010) Nature 465:487.
  10. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  11. Yu, G. et al. (2003) J. Immunol. 171:106.
  12. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  13. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  14. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  15. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  16. Dravis, C. et al. (2004) Dev. Biol. 271:272.
Entrez Gene IDs
1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names
EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1

Citations for Recombinant Zebrafish Ephrin-B2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation.
    Authors: Hendricks M, Mathuru AS, Wang H, Silander O, Kee MZ, Jesuthasan S
    Mol. Cell. Neurosci., 2007-10-16;37(2):271-83.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  2. Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc).
    Authors: D&amp;apos;Souza J, Hendricks M, Le Guyader S, Subburaju S, Grunewald B, Scholich K, Jesuthasan S
    Development, 2004-12-08;132(2):247-56.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Binding Assay

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