Recombinant Viral CKBP Protein

Catalog #: 8254-CK Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
8254-CK-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
8254-CK-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Viral CKBP Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 0.02-0.1 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived viral CKBP protein
Pro18-Val258, with an N-terminal 6-His tag

Accession #

P19063

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

27 kDa

SDS-PAGE

36-40 kDa, reducing conditions

Product Datasheets

8254-CK (with carrier)

Product Datasheet

COA

SDS

8254-CK/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8254-CK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8254-CK/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CKBP

Chemokine-binding Protein (CKBP), also known as, CKBP-II, vCCI, or B29R, is a 35 kDa secreted protein encoded by multiple poxviruses, including the Vaccinia virus (Lister strain) (1). While CKBP binds and sequesters CC chemokines with high affinity, it shares little amino acid sequence identity with CC chemokine receptors (1-3). CKBP prevents CC chemokine activity by masking the chemokine/chemokine receptor binding site (4). Chemokine-binding proteins encoded by poxvirus and herpesvirus strains inhibit the recruitment of immune cells and contribute to viral evasion of host immune responses (5). Local administration of pan-CC chemokine binding proteins such as CKBP have been shown to reduce inflammation resulting from allergic airway hyperactivity and arthritis (6, 7).

References