Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF

Catalog #: 7175-AS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7175-AS-020

Recombinant Yeast ATP‑Sulfurylase/MET3 SDS-PAGE.

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All ATP-Sulfurylase/MET3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant S. cerevisiae ATP Sulfurylase/MET3 Protein, CF Summary

Product Specifications

Endotoxin Level

<1.000 EU per 1 µg of the protein by the LAL method.

Activity

Measured by its ability to sulfurylate ATP.
The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.

Accession #

P08536

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

59 kDa

SDS-PAGE

57-58 kDa, reducing conditions

Product Datasheets

7175-AS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7175-AS

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Yeast ATP‑Sulfurylase/MET3 (Catalog # 7175-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-58 kDa.

Reconstitution Calculator

Background: ATP-Sulfurylase/MET3

Sulfur metabolism is essential to all organisms. Inorganic sulfate is sequentially activated by ATP Sulfurylase and adenosine 5′-phosphosulfate kinase (APSK). ATP Sulfurylase converts ATP to adenosine-5'-phosphosulfate (APS) and generates pyrophosphate (1). APSK subsequently phosphorylates APS at the 3’ site to generate 3'‑phosphoadenosine-5'-phosphosulfate (PAPS) (2). PAPS is the sulfur donor required for all sulfotransferase reactions in humans and is also the sulfur source for synthesis of cysteine, methionine and glutathione (3). The ATP Sulfurylase reaction is highly unfavorable energetically with the standard free-energy change of approximately +46 kJ/mol (4). To drive the reaction forward, APSK and pyrophosphatase are generally added into the reaction simultaneously to remove the products. The recombinant S. cerevisiae ATP Sulfurylase can be used for in vitro PAPS synthesis (5, 6).

References