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Recombinant Rat Reg3A Protein, CF

Catalog #: 8347-RG Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8347-RG-050
Reg3A Induced Cell Proliferation.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Reg3A Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.1-0.6 μg/mL.
Source
E. coli-derived rat Reg3A protein
Glu26-Gln174, with a N-terminal Met
Accession #
BAA04904
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

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8347-RG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8347-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Reg3A Induced Cell Proliferation. View Larger

Recombinant Rat Reg3A (Catalog # 8347-RG) stimulates cell proliferation by RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.1-0.6 μg/mL.

SDS-PAGE Reg3A Induced Cell Proliferation. View Larger

1 μg/lane of Recombinant Rat Reg3A (Catalog # 8347-RG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 16.5 and 16.1 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg3A

Reg3A (Regenerating islet-derived protein 3 alpha), also known as Reg III-alpha, PAP2 (pancreatitis-associated protein 2), and Lithostathine 3 in rats, or HIP/PAP (human islet peptide/PAP) in humans, is a secreted 16-17 kDa type III member of the Reg family, which is the group 7 subfamily of C-type lectins (1, 2). The four type III genes ( alpha -δ) in the rat Reg family are thought to have cell-protective and proliferative effects (2-4). Like other Reg proteins, the 149 amino acid (aa) mature rat mouse Reg3A (aa 26‑174) contains a small, trypsin-cleavable PAP domain (aa 26-36) and a C-type lectin domain (2, 5).  Mature rat Reg3A shares 82% and 58% aa sequence identity with mouse and human Reg3A, (also called HIP/PAP) respectively. Rodent Reg3A is mainly expressed in the intestinal tract and the exocrine pancreas by acinar cells and islet alpha  cells;, and it is up-regulated by IL-6 enhances expression (1, 3, 6, 7). Pancreatic Reg3A expression is also increased in mouse models of type 1 diabetes (4). In the rat brain, nerve injury and inflammation increase Reg3A expression in dorsal root ganglion neurons, while low dopamine levels duringin stress induce its production by melanocytes (8, 9). Treatment of rat macrophages with Reg3A causes up-regulation of NFkB signaling and modulation of cytokine production (2, 10). In humans, Reg3A expression and proteolytic activation in the small intestine areis thought to have a protective effect against infection and TNF-alpha -induced stress (11).

References
  1. Narushima, Y et al. (1997) Gene 185:159.
  2. Viterbo, D. et al. (2008) J. Immunol. 181:1959.
  3. Cui, W. et al. (2009) Growth Factors 27:195.
  4. Lu, Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 291:E50.
  5. Graf, R. et al. (2001) J. Biol. Chem. 276:21028.
  6. Wang, Y. et al. (2011) Growth Factors 29:72.
  7. Gurr, W. et al. (2007) Diabetes 56:34.
  8. He, S.Q. et al. (2010) Mol. Pain 6:23.
  9. Konishi, H. et al. (2011) Biochem. Biophys. Res. Commun. 407:7.
  10. Viterbo, D. et al. (2008) J. Immunol. 181:1948.
  11. Medveczky, P. et al. (2009) Biochem. J. 420:335.
Long Name
Regenerating Islet-derived IIIa
Entrez Gene IDs
5068 (Human); 19694 (Mouse); 171162 (Rat)
Alternate Names
hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III

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