Recombinant Rat RAGE Fc Chimera Protein, CF

Catalog #: 1616-RG
1 Citation Datasheet / COA / SDS

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1616-RG-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat RAGE Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. rrRAGE/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody-coated plate (0.5 µg/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA, Catalog # BT4127) with a linear range of 0.02-15 µg/mL.

Source

Mouse myeloma cell line, NS0-derived rat RAGE protein

Rat RAGE (Gln24 - Ala342) Accession # Q63495	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q63495

N-terminal Sequence
Analysis

No results obtained: Gln24 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

1616-RG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1616-RG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: RAGE/AGER

Advanced glycation endproducts (AGEs) are adducts formed by the non‑enzymatic glycation of macromolecules. AGE formation is accelerated in oxidative and hyperglycemic conditions, diabetes, renal failure, atherosclerosis, Alzheimer’s disease, arthritis, and in normal aging (1 - 5). Receptor for advanced glycation endproducts (RAGE) is a 35 kDa type I transmembrane protein belonging the immunoglobulin superfamily. Besides AGEs, RAGE binds beta -amyloid peptide, S100/calgranulin family proteins, HMGB1/amphoterin, and leukocyte integrins (6 ‑ 9). Mature rat RAGE consists of a 319 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and two Ig-like C-type domains, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (10). Within the ECD, rat RAGE shares 79% and 91% aa sequence identity with human and mouse RAGE, respectively. In human, soluble forms of RAGE are generated by alternate splicing and are associated with multiple disease states (11, 12). RAGE is expressed in the embryonic central nervous system and on macrophages, monocytes, smooth muscle cells, and endothelial cells (13 ‑ 15). It is upregulated in response to AGE accumulation, and its activation induces a broad proinflammatory response (6, 15). The increased production of reactive oxygen species during inflammation promotes additional AGE formation and RAGE upregulation, a cycle that exacerbates diabetic complications and inflammation-induced tissue injury (2, 4).

References

Schleicher, E. and U. Friess (2007) Kidney Int. Suppl. 106:S17.
Herold, K. et al. (2007) J. Leukoc. Biol. 82:204.
Thornalley, P.J. (2006) J. Ren. Nutr. 16:178.
Goldin, A. et al. (2006) Circulation 114:597.
Ramasamy, R. et al. (2005) Glycobiology 15:16R.
Kislinger, T. et al. (1999) J. Biol. Chem. 274:31740.
Yan, S.D. et al. (1996) Nature 382:685.
Huttenen, H. et al. (2000) J. Biol. Chem. 275:40096.
Chavakis, T. et al. (2003) J. Exp. Med. 198:1507.
Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
Yonekura H, et al. (2003) Biochem. J. 370:1097.
Koyama, H. et al. (2007) Mol. Med. 13:625.
Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
Bierhaus, A. et al. (2006) Curr. Opin. Investig. Drugs 7:985.

Long Name

Receptor for Advanced Glycation End Products

Entrez Gene IDs

177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)

Alternate Names

advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

Related Research Areas

Citation for Recombinant Rat RAGE Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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