Recombinant Rat OX40 Ligand/TNFSF4 Protein

Carrier Free

Catalog # Availability Size / Price Qty
8640-OX-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8640-OX-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat OX40 Ligand/TNFSF4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.05-0.25 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived rat OX40 Ligand/TNFSF4 protein
Gln49-Leu199, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
18 kDa
SDS-PAGE
17-31 kDa, reducing conditions

Product Datasheets

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8640-OX (with carrier)

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8640-OX/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8640-OX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8640-OX/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L; also known as TNFSF4, CD252, and gp34) is a type II transmembrane glycoprotein belonging to the TNF superfamily (1-3). OX40 Ligand coordinates with other costimulators (B7, CD40 Ligand/TNFSF5, CD30 Ligand/TNFSF8, CD27 Ligand/TNFSF7, and 4-1BB Ligand/TNFSF9) to manage the activation of the immune response. The rat OX40 Ligand cDNA encodes a 199 amino acid (aa) polypeptide with a short amino-terminal cytoplasmic domain and a 151 aa carboxy-terminal extracellular domain (ECD). The rat OX40 Ligand ECD shares 79% and 40% aa sequence identity with mouse and human OX40 Ligand ECD, respectively. OX40 Ligand is expressed on the surface of activated antigen presenting cells, B cells, T cells, and endothelial cells at sites of inflammation (4-6). Similar to other TNF superfamily members, membrane-bound OX40 Ligand is expressed as a homotrimer (7). OX40 Ligand binds to the type 1 transmembrane receptor, OX40, which is expressed on activated T cells. OX40 Ligand-OX40 signaling results in increased T cell survival, proliferation, and cytokine production (8, 9). It also modulates natural killer cell function, inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs), and promotes the maintenance of and recall response in memory T cells (7-13). OX40 Ligand-OX40 signaling is involved in allergic airway inflammation, graft-versus-host disease, and other autoimmune diseases (14-16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (13, 17).

References
  1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.
  2. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
  3. Baum, P.R. et al. (1994) Circ. Shock 44:30.
  4. Gough, M.J. and A.D. Weinberg (2009) Adv. Exp. Med. Biol. 647:94.
  5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
  6. Ohshima, Y. et al. (1997) J. Immunol. 159:3838.
  7. Croft, M. (2010) Annu. Rev. Immunol. 28:57.
  8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
  9. Xiao, X. et al. (2008) J. Immunol. 181:3193.
  10. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
  11. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  12. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  13. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
  14. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
  15. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
  16. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
Entrez Gene IDs
7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1

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