Recombinant Rat NKp30 Fc Chimera Protein, CF

Catalog #: 10057-NK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10057-NK-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat NKp30 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat NKp30/NCR3 Fc Chimera is immobilized at 5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human B7-H6 Fc Chimera (Catalog # BT7144) that produces 50% of the optimal binding response is 1-6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat NKp30/NCR3 protein

Rat NKp30 (Val22-Ser150) Accession # NP_861543.2	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_861543.2

N-terminal Sequence
Analysis

Val22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

42-64 kDa, reducing conditions

Product Datasheets

10057-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10057-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NKp30/NCR3

Natural killer cell p30-related protein (NKp30), also known as Natural cytotoxicity triggering receptor 3 (NCR3), is a type I transmembrane glycoprotein consisting of an extracellular domain (ECD) containing a single V-like immunoglobulin domain, a transmembrane domain containing a positively-charged amino acid (aa) residue, and a short cytoplasmic tail (1). NKp30, along with NKp44/NCR2 and NKp46/NCR1, is a member of Natural Cytotoxicity Receptor (NCR) group of proteins, which play a major role in triggering NK-mediated lysis of tumor cells (2). Although a pseudogene in mice, the ECD of mature rat NKp30 shares 68% aa identity with human NKp30.

In rats, NKp30 is expressed on a subset of NK cells present in blood, spleen, and, to a lesser extent, liver. These tissues also express different percentages and levels of NKp30, which is a departure from the other NK activation receptors which are expressed constitutively (3). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (1, 5). NKp30 has been found to bind to the human protein B7-H6 (6). B7-H6 binding activates the NKp30 pathway leading to cytokine release and cytotoxic function against target cells. B7-H6 is more highly expressed on tumors than on healthy tissue, and thus functions in antitumor immunosurveillance (7). Our data shows that rat NKp30 binds to human B7-H6.

References

Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Moretta, L. and Moretta, A. (2004) EMBO J. 23:255.
Hsieh, C. et al. (2006) Eur. J. Immunol. 36:2170.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Brandt, C. et al. (2009) J. Exp Med. 206:1495.
Textor, S. et al. (2016) Oncoimmunology. 5:e1116674.

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Alternate Names

Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS