Recombinant Rat MMR/CD206 Protein, CF

Catalog #: 7858-MR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7858-MR-050

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Recombinant Rat MMR/CD206 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Mannan is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Rat MMR/CD206 that produces 50% optimal binding response is 0.1-0.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat MMR/CD206 protein
Leu26-Ala1395, with a C-terminal 6-His tag

Accession #

NP_001099593

N-terminal Sequence
Analysis

Starts at Leu26

Predicted Molecular Mass

156.4 kDa

SDS-PAGE

150-180 kDa, reducing conditions

Product Datasheets

7858-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7858-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C-type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor-associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1463 amino acid (aa) rat CD206 precursor contains a signal sequence (26 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II (FNII) domain, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The rat CD206 ECD shares 96% aa sequence identity with mouse CD206, and 84‑85% with human, equine, porcine and canine CD206. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

References

Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
Harris, N. et al. (1992) Blood 80:2363.
Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
Chieppa, M. et al. (2003) J. Immunol. 171:4552.
Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Long Name

Macrophage Mannose Receptor

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Alternate Names

CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1