Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

Catalog #: 8897-LR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8897-LR-050

R&D Systems Recombinant Proteins and Enzymes

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All Lymphotoxin beta R/TNFRSF3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Lymphotoxin alpha 1/ beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat Lymphotoxin beta R/TNFRSF3 protein

Rat Lymphotoxin beta R (Gln29-Met222) Accession # NP_001008316	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_001008316

N-terminal Sequence
Analysis

No results obtained. Gln29 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

49 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Product Datasheets

8897-LR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8897-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta R (LT beta R; also called TNFRSF3, TNF RIII,or TNF Rrp) is a type I transmembrane glycoprotein within the TNF receptor superfamily (1-3). The rat LT beta R cDNA encodes a 415 amino acid (aa) protein that includes a 30 aa signal peptide, a 191 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine-rich motifs characteristic of the TNF receptor superfamily (2). Within the ECD, rat LT beta R shares 90% and 66% aa sequence identity with the ECD of mouse and human LT beta R, respectively. Soluble LT beta R, which can be formed by proteolytic cleavage of the ECD, functions as a decoy receptor for LT beta R ligands and is involved in the suppression of autoimmunity (4, 5). LT beta R is constitutively expressed in cells of myeloid lineage and is upregulated during tissue regeneration (6). It is expressed on mesenchymal stromal organizing cells that give rise to primary, secondary, and tertiary lymphoid structures (7-9). Mice deficient in LT beta R fail to form secondary lymphoid structures (4). LT beta R ligands include homotrimers of LIGHT/TNFSF14 and the heterotrimeric Lymphotoxin alpha 1/ beta 2 (2). Ligand engagement of LT beta R has been shown to induce NF kappa B activation through canonical (IKK) or alternative (NIK/RelB) signaling pathways (5, 10). LT beta R signaling induces production of cytokines (TRANCE/RANK Ligand/TNFSF11, IL-7), chemokines (CXCL8/IL-8, CXCL13/BCL/BCA-1, CCL19/MIP-3 beta, CCL21/6Ckine, CCL2/MCP-1), and adhesion molecules (VCAM-1/CD106, ICAM-1/CD54, MAdCAM) (8, 11). LT beta R is involved in lipid metabolism, atherosclerosis, and intestinal epithelial homeostasis (4, 12, 13). It also regulates cell growth and can initiate inflammation-related carcinogenesis (5, 14).

References

Force, W.R. et al. (1995) J. Immunol. 155:5280.
Remouchamps, C. et al. (2011) Cytokine Growth Factor Rev. 22:301.
Crowe, P.D. et al. (1994) Science 264:707.
Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
Wolf, M.J. et al. (2010) Oncogene 29:5006.
Ware, C.F. (2005) Annu. Rev. Immunol. 23:787.
Boulianne, B. et al. (2012) Front. Immunol. 3:243.
Mouri, Y. et al. (2011) J. Immunol. 186:5047.
van de Pavert, S.A. and R.E. Mebius (2010) Nat. Rev. Immunol. 10:664.
Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
Mikami, Y. et al. (2014) PLoS One 9:e114791.
Macho-Fernandez, E. et al. (2015) Mucosal Immunol. 8:403.
Browning, J.L. (2012) Mucosal Immunol. 5:228.
Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Long Name

Lymphotoxin beta Receptor

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Alternate Names

CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III