Recombinant Rat IL-22 Protein

Catalog #: 1316-RL
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1316-RL-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1316-RL-010

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (3)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-22 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679. The ED₅₀ for this effect is 150-750 pg/mL.

Source

E. coli-derived rat IL-22 protein
Leu27-Val172, with an N-terminal Met

Accession #

Q198B4

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

16.7 kDa

Product Datasheets

1316-RL (with carrier)

Product Datasheet

COA

SDS

1316-RL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1316-RL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1316-RL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF), was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. It belongs to the IL-10-related cytokine family that consists of six members (IL-10, IL-19, IL-20, IL-22, IL-24/MDA-7, and IL-26/AK155). These proteins share structural homology and some degree of amino acid sequence homology to IL-10. Receptors for these proteins are members of the class II cytokine receptor family (1). The rat IL-22 coding region corresponding to amino acids 48 - 179 was deduced from a rat genomic clone (Genbank accession numaber AC111483). The N-terminal portion was cloned from rat adipocyte first strands using degenerate forward primers based on the human and mouse IL-22 amino acid sequences in two independent PCR reactions (2). Rat IL-22 cDNA predicts a 179 amino acid (aa) residue precursor protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein, which shares approximately 92% and 79% aa sequence identity with mouse and human IL-22, respectively. IL-22 signals through a heterodimeric receptor complex composed of the IL-22R (CRF2-9) subunit and the ( chain of IL-10R (CRF2-4). In addition, IL-22 also binds to a secreted member of the class II cytokine receptor family called IL-22BP that acts as a natural IL-22 antagonist. IL-22 upregulates acute-phase reactants in the liver and hepatoma cells. In a rat hepatoma cell line, IL-22 has been shown to activate the Jak/STAT and MAPK signaling pathways (3).

References

Kotenko, S.V. (2002) Cytokine and Growth Factor Reviews 13:223.
Kettlewell, J. (2002) R&D Systems, unpublished results.
Lejeune, D. et al. (2002) J. Biol. Chem. 277:33673.

Long Name

Interleukin 22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Alternate Names

Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

Related Research Areas

Citations for Recombinant Rat IL-22 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Intestinal IL-22RA1 signaling regulates intrinsic and systemic lipid and glucose metabolism to alleviate obesity-associated disorders
Authors: Gaudino, SJ;Singh, A;Huang, H;Padiadpu, J;Jean-Pierre, M;Kempen, C;Bahadur, T;Shiomitsu, K;Blumberg, R;Shroyer, KR;Beyaz, S;Shulzhenko, N;Morgun, A;Kumar, P;
Nature communications
Species: Mouse
Sample Types: Organoid
Applications: Bioassay
IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis
Authors: H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson
J. Immunol., 2019-07-10;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture
Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin
Sci Rep, 2016-11-07;6(0):36436.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo