Recombinant Rat FGF-BP Protein

Catalog #: 1413-FB
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1413-FB-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1413-FB-025

R&D Systems Recombinant Proteins and Enzymes

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat FGF-BP Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhFGF-basic at 5 µg/mL (100 µL/well) can bind rrFGF-BP with a linear range of 0.8-50 ng/mL.

Source

Mouse myeloma cell line, NS0-derived rat FGF-BP protein
Glu21-Cys238, with a C-terminal Asp and 10-His tag

Accession #

Q9QY10

N-terminal Sequence
Analysis

Glu21

Predicted Molecular Mass

26 kDa

SDS-PAGE

Multiple bands between 25-37 kDa, reducing conditions

Product Datasheets

1413-FB (with carrier)

Product Datasheet

COA

SDS

1413-FB/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1413-FB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1413-FB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2 - 4). FGF-BP contains a heparin-binding domain (aa 113 - 146) and a distinct FGF-binding region (aa 197 - 238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8 - 11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8 - 10, 12, 15, 16).

References

Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
Aigner, A. et al. (2000) Growth Factors 18:51.
Tassi, E. et al. (2006) Cancer Res. 66:1191.
Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
Beer, H.-D. et al. (2005) Oncogene 24:5269.
Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
Kurtz, A. et al. (2004) Neoplasia 6:595.
Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
Kurtz, A. et al. (1997) Oncogene 14:2671.
Aigner, A. et al. (2001) Int. J. Cancer 92:510.
Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Alternate Names

17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein

Related Research Areas

Citation for Recombinant Rat FGF-BP Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1