Recombinant Rat Fc gamma RI/CD64 Protein, CF

Catalog #: 9558-FC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9558-FC-100

Recombinant Rat Fc gamma RI/CD64 Protein Binding Activity

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All Fc gamma RI/CD64 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Fc gamma RI/CD64 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat Fc gamma RI/CD64 protein
Gln16-Pro285, with a C-terminal 6-His tag

Accession #

NP_001094306

N-terminal Sequence
Analysis

Thr21 observed & Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17

Predicted Molecular Mass

31 kDa

SDS-PAGE

36-48 kDa, reducing conditions

Product Datasheets

9558-FC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9558-FC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Rat Fc gamma RI/CD64 (Catalog # 9558‑FC) is immobilized at 1 μg/mL, 100 μL/well, Biotinylated Rat IgG binds with an ED₅₀ of 0.075-0.35 μg/mL.

Reconstitution Calculator

Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). The CD64 proteins are high affinity receptors (~10^-8-10^-9 M) that are capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^-6-10^-7 M) (4, 5). The high affinity of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune response (6). The mature protein from this family consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fcɣ RI shares 78% and 64% amino acid sequence identity with mouse and human Fc gamma RI, respectively. Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils. Its expression is up-regulated during bacterial infections and sepsis (1, 5).

References

Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
Chenoweth, A.M et al. (2015) Immunol. Rev. 268:175.
Takai, T. (2002) Nature Rev. Immunol. 2:580.
Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.

Long Name

Fc gamma Receptor I

Entrez Gene IDs

2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)

Alternate Names

CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I