Recombinant Rat CD40/TNFRSF5 Fc Chimera Protein, CF

Catalog #: 8229-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8229-CD-050

Recombinant Rat CD40/TNFRSF5 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CD40/TNFRSF5 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit CD40 Ligand-induced proliferation of mouse B cells. The ED₅₀ for this effect is 0.1-0.6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat CD40/TNFRSF5 protein

Rat CD40 (Val24-Arg193) Accession # Q4QQW2	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q4QQW2

N-terminal Sequence
Analysis

Val24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

57-63 kDa, reducing conditions

Product Datasheets

8229-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8229-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Rat CD40/TNFRSF5 Fc Chimera (Catalog # 8229-CD) inhibits CD40 Ligand-induced proliferation of mouse B cells. The ED₅₀ for this effect is 0.1-0.6 μg/mL.

Reconstitution Calculator

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is an approximately 32-35 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. The extracellular domain of rat CD40 shares 81% and 56% aa sequence identity with mouse and human CD40, respectively. Multiple rat CD40 splice variants have been reported including soluble isoforms. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (1, 2). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of intracellular signaling in both CD40 and CD40 Ligand expressing cells (3). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (4, 5). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (6, 7). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (8, 9). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (10, 11).

References

van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
Pamukcu, B. et al. (2011) Ann. Med. 43:331.
Hassan, G.S. et al. (2012) Immunobiology 217:521.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5