Recombinant Rat B7-H2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10419-B7-100
Recombinant Rat B7-H2 Fc Chimera Protein Binding Activity
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Recombinant Rat B7-H2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is Immobilized at 1.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) that produces 50% optimal binding response is found to be approximately 10-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived rat B7-H2 protein
Rat B7-H2
(Glu25-Lys261)
Accession # XP_006256322.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Glu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass

54 kDa

SDS-PAGE
70-90 kDa, under reducing conditions

Product Datasheets

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10419-B7

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10419-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Rat B7-H2 Fc Chimera Protein Binding Activity View Larger

When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is immobilized at 1.0 µg/mL (100 µL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) that produces 50% the optimal binding response is found to be approximately 10-100 ng/mL.

SDS-PAGE Recombinant Rat B7-H2 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-90 kDa and 140-180 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H2

B7-H2, also known as B7-related protein 1 (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa type I transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Within the extracellular domain, rat B7-H2 shares 70% and 54% amino acid (aa) sequence identity with mouse and human B7‑H2, respectively. Alternative splicing generates a long isoform that carries a 10 aa substitution for the 3 C-terminal residues in humans and a 27 aa substitution for the 2 C-terminal residues in mouse. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). B7-H2 binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Rat and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14). In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thyroid hormones (15). B7-H2 and ICOS are also expressed on ILC2 cells. B7-H2/ICOS interaction promoted cytokine production and survival in ILC2 cells through STAT5, suggesting that B7-H2/ICOS signaling pathway is critically involved in ILC2 function and homeostasis (16).

References
  1. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  2. Wang, S. et al. (2000) Blood 96:2808.
  3. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
  4. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  5. Faget, J. et al. (2012) Cancer Res. 72:6130.
  6. Yao, S. et al. (2011) Immunity 32:729.
  7. Watanabe, M. et al. (2008) J. Immunol. 180:5222.
  8. Wong, S.-C. et al. (2003) Blood 102:1381.
  9. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
  10. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
  11. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
  12. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
  13. Akbari, O. et al. (2002) Nat. Med. 8:1024.
  14. Her, M. et al. (2009) Lupus 18:501.
  15. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
  16. Maazi H. et al. (2015) Immunity. 42:538.
Long Name
B7 Homolog 2
Entrez Gene IDs
23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand

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