Recombinant Porcine CD44 Fc Chimera Protein, CF

Catalog #: 7189-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7189-CD-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine CD44 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Porcine CD44 Fc Chimera is present at 1 μg/mL, the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 4-20 ng/mL.

Source

Mouse myeloma cell line, NS0-derived porcine CD44 protein

Porcine CD44 (Gln33 - Thr234) Accession # XP_003353954	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

XP_003353954

N-terminal Sequence
Analysis

Gln33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48.7 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Product Datasheets

7189-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7189-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1 ‑ 5). Porcine CD44 is a type I transmembrane protein with a variable‑length extracellular domain (ECD) that contains a 100 amino acid (aa) hyaluronan‑binding disulfide‑stabilized link region and a 48 ‑ 463 aa stem region. Within the stem, variably spliced exons produce multiple protein isoforms (1 ‑ 5). The shortest form does not include the variable segments, and is called the standard or hematopoietic form in humans (1 ‑ 5). In humans, cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (2, 4). With variable N- and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1, 2). Within the ECD of CD44, porcine CD44 (aa 33 ‑ 234) shares 74 ‑ 81% aa sequence identity with the corresponding mouse, human and rat CD44 sequence. The many reported functions of CD44 fall within three categories (1, 2). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 is a co‑receptor that modifies activity of receptors including MET and the ErbB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane‑type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (6, 7). The cellular portion may then undergo gamma secretase‑dependent intramembrane cleavage to form an A beta ‑like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (8, 9). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 2, 6). In pigs, CD44 expressed on cumulus cells plays a key role in oocyte maturation through hyaluronan binding in the cumulus‑oocyte complex (10).

References

Pure, E. and R.K. Assoian (2009) Cell. Signal. 21:651.
Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
Nakamura, H. et al. (2004) Cancer Res. 64:876.
Murakami, D. et al. (2003) Oncogene 22:1511.
Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Yokoo, M. et al. (2007) J. Reprod. Dev. 53:263.

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Alternate Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I