Recombinant Porcine CD31/PECAM-1 Protein, CF

Catalog #: 3387-PC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3387-PC-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine CD31/PECAM-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 10⁴ cells/well are added to PECAM-1 coated plates (4 µg/mL, 100 µL/well), approximately 45-65% will adhere after 10-20 minutes at 37° C.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived porcine CD31/PECAM-1 protein
Gln28-Lys602, with a C-terminal 6-His tag

Accession #

Q95242

N-terminal Sequence
Analysis

No results obtained: Gln28 predicted

Predicted Molecular Mass

65 kDa

SDS-PAGE

100-115 kDa, reducing conditions

Product Datasheets

3387-PC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3387-PC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD31/PECAM-1

CD31, also known as PECAM-1 (platelet-endothelial cell adhesion molecule-1), is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to the vascular system, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1 - 3). The extracellular domain (ECD) of CD31 has ten potential N-glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Porcine CD31 ECD shows 74%, 73%, 70%, 63% and 62% amino acid (aa) identity with bovine, canine, human, mouse and rat CD31, respectively. CD31 participates with other adhesion molecules for most functions but is the critical molecule for TEM. Homotypic CD31 adhesion in trans combined with cycling of CD31 to and from surface-connected endothelial cell vesicles leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31^-/- mice are deficient in chemokine-mediated chemotaxis (14).

References

Ilan, N. and J.A. Madri (2003) Curr Opin. Cell Biol. 15:515.
Nasu, K. et al. (1999) Transplantation 68:861.
Liao, F. et al. (1997) J. Exp. Med. 185:1349.
Nakada, M.T. et al. (2000) J. Immunol. 164:452.
Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
Ilan, N. et al. (2001) FASEB J. 15:362.
Eugenin, E.A. et al. (2006)J. Leukoc. Biol. 79:444.
Losy, J. et al. (1999) 99:169.
Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
Mamdouh, Z. et al. (2003) Nature 421:748.
Gao, C. et al. (2003) Blood 102:169.
Falati, S. et al. (2006) Blood 107:535.
Wee, J. L. and D.E. Jackson (2005) Blood 106:3816.
Wu, Y. et al. (2005) J. Immunol. 175:3484.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Alternate Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule