Recombinant P. humanus PINK1 Protein, CF

Name: Recombinant P. humanus PINK1 Protein, CF
Brand: R&D Systems
SKU: AP-182

Catalog #: AP-182 Datasheet / COA / SDS

Please inquire regarding future lot manufacture.

Will be discontinued when existing inventory is gone

Catalog #	Availability	Size / Price	Qty
AP-182-100

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Recombinant P. humanus PINK1 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial PINK1 concentration of 0.5-2 µM for the phosphorylation of recombinant Parkin, Ubiquitin, or Polyubiquitin chains.

Source

E. coli-derived p. humanus PINK1 protein
Trp129 - Asn575 with a N-terminal GST tag

Accession #

XP_002432225.1

Predicted Molecular Mass

80 kDa

Product Datasheets

AP-182

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-182

Formulation	Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: PINK1

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by 1) PINK-mediated phosphorylation of PARK2 at serine 65, and 2) PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Recombinant human PINK1 is not active in vitro, while this protein from the Human Body Louse (Pediculus humanus) effectively phosphorylates recombinant Parkin, mono-Ubiquitin, and poly-Ubiquitin chains.

References

Kane, L.A., et al. (2014) J. Cell Biol. 205:143.
Matsuda, N., et al. (2010) J. Cell Biol. 189:211.
Vives-Bauza, C., et al. (2010) Proc. Natl. Acad. Sci. 107:378.
Wauer, T., et al. (2015) EMBO J. 34:307.

Long Name

PTEN-induced Putative Kinase 1

Entrez Gene IDs

65018 (Human); 68943 (Mouse); 298575 (Rat); 3925520 (T. castaneum)

Alternate Names

BRPK; EC 2.7.11.1; FLJ27236; PARK6; Parkinson disease (autosomal recessive) 6; PINK1; protein kinase BRPK; PTEN Induced Kinase 1; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; serine/threonine-protein kinase PINK1, mitochondrial